[Photometric and chromatographic methods of measuring the concentration of acelysin in the air of a work area].

Abstract

Two methods were applied to evaluation of acelysine level. Spectrophotometric one was based on reaction with p-nitrophenyldiasonium and evaluation of the product optic density at 510 nm. The second was chromatography in thin layer, where a mixture of n-butanol, formic acid and water was used as an eluent and 0.2% ninhydrin solution in butanol and 3 ml of ice acetic acid (60-70 degrees C) served as a developer. Both methods are supposed to evaluate acelysine level in the air of working area, when a half of MAC and admixtures are present.