Highly Sensitive Protein Detection Using Conductive Polymer Nanowires Fabricated by Nanoscale Soft Lithography

Abstract

Nanowires constructed as biosensors are used for protein detection with high sensitivity recently. However, they still suffer a high fabrication cost and the device repeatability issues. Here, we demonstrate fabrication of conductive polymer nanowires based biosensor by low cost nanoscale soft lithography. Poly(3,4-ethylenedioxythiophene)-poly(styrene-sulfonate) (PEDOT:PSS) doped with biotin-derivatized poly(L-lysine)-grafted oligo-ethylene glycol (PLL-g-OEG4-Biotin) copolymers were patterned on silicon substrate by PDMS nanomold and further constructed as electrochemical biosensor. As shown from the result, the detection limit of proposed nanoscale biosensor for streptavidin is as low as 1 fg/ml.