Medium selection in tissue culture of japonica rice cultivar Nipponbare and preparation of transgenic plants with an elicitor-coding gene from Magnaporthe grisea

Abstract

N6, MS and BN mediums were adopted for tissue culture of Nipponbare (japanica) scutellum, and the effects of the three mediums on the cultrue were compared. Results indicated that, among the three kinds of culture mediums, NB medium worked best in the calli induction and gene transformation and was most suitable for tissue culture of Nipponbare scutellum. Based on this result, elicitor-encoding gene pemG1 from Magnaporthe grisea was introduced into the genome of Nipponbare via Agrobacterium-mediated method. And transgenic rice plants were obtained. The integration, transcription and expression of pemG1 in rice were confirmed by PCR, Northern blot and Western blot, respectively. Genetic analysis showed that the transgenes were segregated normally in the progenies.