Convection-Enhanced Delivery of Alkaloid - Loaded Maghemite Nanoparticles against 9L - Gliomass Cell Line

Abstract

Convection Enhanced Delivery (CED) is a novel approach to synthesize and facilitate targeted delivery of phytocontituents/ pharmaceuticals to the brain. The CED procedure involves a minimally invasive surgical exposure of the brain, followed by placement of small diameter catheters directly into the brain tumor. The study was designed to synthesize unique, sustained release, targeted delivery with no toxicity of piperidine alkaloids along with maghemite (γ-Fe2O3), poly-ethylene glycol and human serum albumin conjugation using 9L-gliomass cell line. Subsequently different nanoparticles (NPs) were prepared Naked NPs and HSA- or polyethylene glycol (PEG)-coated NPs with/without piperidine alkaloid were studied. In vitro results showed no toxicity and a similar cell-kill efficacy of the piperidine alkaloid-loaded particles via HSA coating to that of free piperidine alkaloid, while piperidine alkaloid-loaded particles via PEG coating showed low efficacy