ĐƯỜNG KÍNH SỢI FIBRIN VÀ ĐỘ XỐP CỦA A-PRF+ DƯỚI KÍNH HIỂN VI ĐIỆN TỬ QUÉT

Phong Lâm Cự, Ngọc Nguyễn Thị Bảo, Lý Nguyễn Thị Bích
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Abstract

Objectives: The aim of this study is to evaluate mean fibrin fiber diameter and porosity of PRF under scanning electronic microscope. Methods: A descriptive study on 10 patients who were willing to have PRF placement in tooth socket after third molar extraction. 20 ml of blood was obtained from each patient within 2 blood collecting tube. The blood samples were then centrifuged with A-PRF+ mode (Duo Quattro PRF system). One PRF block was placed into tooth socket right after third molar exraction. The other one was cut into 3 parts from plasma end to red blood cells end. Those PRF parts were fixed and underwent preparation procedure for scanning electronic microscope evaluation. Results: From plasma end to red blood cells end, fibrin fiber diameter were 198.519±71.655 nm, 197.519±74.625 nm, 194.270±78.710 nm, PRF porosity were 11.317±2.699%, 10.824 ± 3.610%, 10.521 ± 3.238%, respectively. Fibrin fiber diameter and PRF porosity of a whole PRF block is 196.774±74.590 nm and 10.891±3.144%. The difference of fibrin fiber diameter and PRF porosity in diferrent parts were not statistically significant. Conclusion: PRF block were centrifuged with low-speed centrifugation concept (A-PRF+ mode) is relatively homogeneous in fibrin fiber diameter and PRF porosity. Keywords: third molar extraction, scanning electronic microscope, fibrin fiber diameter, PRF porosity
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A-PRF+在扫描电子显微镜下的纤维直径和多孔性
目的:在扫描电镜下评价PRF的平均纤维蛋白纤维直径和孔隙率。方法:对10例第三磨牙拔牙后愿意在牙槽内放置PRF的患者进行描述性研究。每例患者2根采血管取血20 ml。然后用A-PRF+模式(Duo Quattro PRF系统)离心血样。第三磨牙拔除后,将一个PRF块置入牙槽内。另一个从血浆端到红细胞端切成三段。将这些PRF部件固定并进行扫描电镜评价的准备程序。结果:血浆端至红细胞端,纤维蛋白纤维直径分别为198.519±71.655 nm、197.519±74.625 nm、194.270±78.710 nm, PRF孔隙率分别为11.317±2.699%、10.824±3.610%、10.521±3.238%。整个PRF块的纤维纤维直径和PRF孔隙率分别为196.774±74.590 nm和10.891±3.144%。不同部位纤维蛋白纤维直径和PRF孔隙率差异无统计学意义。结论:采用低速离心概念(A-PRF+模式)离心的PRF块在纤维蛋白纤维直径和PRF孔隙度上相对均匀。关键词:第三摩尔萃取,扫描电镜,纤维蛋白纤维直径,PRF孔隙率
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