[RFLP analysis of single hairs].

Beitrage zur gerichtlichen Medizin Pub Date : 1992-01-01
H J Weisser, R Scheithauer
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Abstract

Investigations were performed with the root of one hair torn out 6 times in parallel. Yield of DNA was about the same with 6 extraction methods tested. Hairs stored at RT up to 8 weeks in non sterile environment showed the same result as deeply frozen hairs. Hairs of one test person could not be typed in repeated investigations, whereas hairs of another person could successfully be typed in every case (n > 100). 35 hairs of 66 samples investigated from 11 other persons could be typed.

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[单根毛发的RFLP分析]。
在进行调查时,将一根头发的根部平行拔出6次。6种提取方法的DNA提取率基本一致。在非无菌环境中保存8周的头发与深度冷冻的头发结果相同。在重复调查中,一个被试者的毛发不能被分类,而另一个被试者的毛发在每次调查中都能成功分类(n > 100)。来自另外11个人的66个样本中的35根头发可以被分类。
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[Aging processes in the brain]. [RFLP analysis of single hairs]. [Population genetic Hae III/RFLP and HLA-DQ-alpha data of a Caucasian population sample in Switzerland]. [Conversion of forensic paternity determination to DNA analysis]. [Effects of nonoxinol 9 on RFLP typing of postcoital vaginal smears].
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