Deep sequence representation learning for predicting human proteins with liquid-liquid phase separation propensity and synaptic functions

Abstract

With advancements in next-generation sequencing techniques, the whole protein sequence repertoire has increased to a great extent. In the meantime, deep learning techniques have promoted the development of computational methods to interpret large-scale proteomic data and facilitate functional studies of proteins. Inferring properties from protein amino acid sequences has been a long-standing problem in Bioinformatics. Extensive studies have successfully applied natural language processing (NLP) techniques for the representation learning of protein sequences. In this paper, we applied the deep sequence model - UDSMProt, to fine-tune and evaluate two protein prediction tasks: (1) predict proteins with liquid-liquid phase separation propensity and (2) predict synaptic proteins. Our results have shown that, without prior domain knowledge and only based on protein sequences, the fine-tuned language models achieved high classification accuracies and outperformed baseline models using compositional k-mer features in both tasks. Hence, it is promising to apply the protein language model to some learning tasks and the fine-tuned models can be used to predict protein candidates for biological studies.