Ayano Kitamura, M. Komatsu, Y. Ohki, M. Mizuno, M. Hirabayashi, H. Kojima
{"title":"Estimation of the number of nucleotides with thymine in deoxyribonucleic acid by far-infrared absorption","authors":"Ayano Kitamura, M. Komatsu, Y. Ohki, M. Mizuno, M. Hirabayashi, H. Kojima","doi":"10.1109/CEIDP.2015.7351989","DOIUrl":null,"url":null,"abstract":"A new research trend that regards deoxyribonucleic acid (DNA) as a new dielectric polymeric material has been emerging in recent years. Especially for such application purposes, a simple easy-to-use method for determining the higher-order structure of synthetic DNA is desirable. From this viewpoint, we demonstrated in this research that far-infrared absorption spectroscopy can be a tool to determine the DNA higher-order structure and we calculated experimentally the integrated molar absorption coefficients per nucleotide with thymine at various frequencies. Therefore, using these coefficients, the number of thymine base on a single stranded DNA can be estimated by far-infrared spectroscopy.","PeriodicalId":432404,"journal":{"name":"2015 IEEE Conference on Electrical Insulation and Dielectric Phenomena (CEIDP)","volume":"47 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2015-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"2015 IEEE Conference on Electrical Insulation and Dielectric Phenomena (CEIDP)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/CEIDP.2015.7351989","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1
Abstract
A new research trend that regards deoxyribonucleic acid (DNA) as a new dielectric polymeric material has been emerging in recent years. Especially for such application purposes, a simple easy-to-use method for determining the higher-order structure of synthetic DNA is desirable. From this viewpoint, we demonstrated in this research that far-infrared absorption spectroscopy can be a tool to determine the DNA higher-order structure and we calculated experimentally the integrated molar absorption coefficients per nucleotide with thymine at various frequencies. Therefore, using these coefficients, the number of thymine base on a single stranded DNA can be estimated by far-infrared spectroscopy.