Estimation of the number of nucleotides with thymine in deoxyribonucleic acid by far-infrared absorption

Ayano Kitamura, M. Komatsu, Y. Ohki, M. Mizuno, M. Hirabayashi, H. Kojima
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引用次数: 1

Abstract

A new research trend that regards deoxyribonucleic acid (DNA) as a new dielectric polymeric material has been emerging in recent years. Especially for such application purposes, a simple easy-to-use method for determining the higher-order structure of synthetic DNA is desirable. From this viewpoint, we demonstrated in this research that far-infrared absorption spectroscopy can be a tool to determine the DNA higher-order structure and we calculated experimentally the integrated molar absorption coefficients per nucleotide with thymine at various frequencies. Therefore, using these coefficients, the number of thymine base on a single stranded DNA can be estimated by far-infrared spectroscopy.
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用远红外吸收法估计脱氧核糖核酸中胸腺嘧啶核苷酸的数目
近年来出现了将脱氧核糖核酸(DNA)作为一种新型介电聚合物材料的研究新趋势。特别是对于这样的应用目的,需要一种简单易用的方法来确定合成DNA的高阶结构。从这个角度出发,我们在本研究中证明了远红外吸收光谱可以作为确定DNA高阶结构的工具,并通过实验计算了胸腺嘧啶在不同频率下每个核苷酸的综合摩尔吸收系数。因此,利用这些系数,可以通过远红外光谱估计单链DNA上胸腺嘧啶碱基的数量。
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