Homayoon Soleimani Dinani, Mehrab Pourmadadi, H. Rashedi, F. Yazdian
{"title":"Fabrication of nanomaterial-based biosensor for measurement of a microRNA involved in cancer","authors":"Homayoon Soleimani Dinani, Mehrab Pourmadadi, H. Rashedi, F. Yazdian","doi":"10.1109/ICBME51989.2020.9319450","DOIUrl":null,"url":null,"abstract":"An electrochemical nanobiosensor is described for the voltammetric determination of miRNA-128 as the cancer biomarker. Aptamer chains were immobilized on the surface of a glassy carbon electrode (GCE) via gold nanoparticles/magnetite/reduced graphene oxide (AuNPs/Fe3O4/RGO). FTIR and XRD analysis were used for the characterization of synthesized nanomaterials. Square wave voltammetry (SWV) was used to characterize the modified GCE in a label-free method. The results indicate that the modified working electrode has high selectivity and for miRNA-128 over other biomolecules. The hexacyanoferrate redox system, typically operated at around 0.3 V (vs. Ag/AgCl) was used as an electrochemical probe. The limit of detection (LOD) and linear detection range are 0.05346 fM and 0.1 to 0.9 fM, respectively.","PeriodicalId":120969,"journal":{"name":"2020 27th National and 5th International Iranian Conference on Biomedical Engineering (ICBME)","volume":"65 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2020-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"10","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"2020 27th National and 5th International Iranian Conference on Biomedical Engineering (ICBME)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/ICBME51989.2020.9319450","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 10
Abstract
An electrochemical nanobiosensor is described for the voltammetric determination of miRNA-128 as the cancer biomarker. Aptamer chains were immobilized on the surface of a glassy carbon electrode (GCE) via gold nanoparticles/magnetite/reduced graphene oxide (AuNPs/Fe3O4/RGO). FTIR and XRD analysis were used for the characterization of synthesized nanomaterials. Square wave voltammetry (SWV) was used to characterize the modified GCE in a label-free method. The results indicate that the modified working electrode has high selectivity and for miRNA-128 over other biomolecules. The hexacyanoferrate redox system, typically operated at around 0.3 V (vs. Ag/AgCl) was used as an electrochemical probe. The limit of detection (LOD) and linear detection range are 0.05346 fM and 0.1 to 0.9 fM, respectively.
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