Physical and biochemical profile of fox-gestational hybrids by sex and age

U. Berezina, M. Kochurnikova, O. Bespyatykh, I. Domskiy
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Abstract

Purpose: The study of biochemical indicators of blood of females and males of fox hybrids with age.Materials and methods. Research was carried out on females and males of fox hybrids. The age dynamics of changes in the biochemical parameters of the blood was evaluated with a comparative analysis of 2 age groups: females and males of fox hybrids 2 and 5 months (n = 30). The animals were fed once a day (in the morning) with meat mixtures in accordance with age and physiological condition, contained under the same conditions. Blood from the animals was taken into a special test tube with an activator of a clot of lateral subcutaneous vein of the lower leg until morning feeding. They received serum from it by centrifugation at 2000 rpm for 15 minutes. To obtain information about the functional state of the body, biochemical tests were selected that reliably reflect the state of metabolic processes as carbohydrate, lipid protein: general protein, albumins, alanineaminotransferase, aspartaminotransferase, alkaline phosphatase, α-amylase, glucose, urea, creatinine.Results. Animals in the process of growth and development showed an increase in a number of indicators: urea, creatinine, total protein, albumin and glucose. ALT and AST activity, on the contrary, decreased with age, reaching minimum values 2 in 5-month-old animals. Alkaline phosphatase concentration also decreased with age. The level of α amylase during the growth and development of the animal did not undergo significant changes. Indicators of protein, carbohydrate metabolism, as well as renal markers and enzymes in fox-bearing hybrids are characterized by sex differences. Thus, the biochemical composition of the blood during the growth and development of the animal undergoes a number of changes. The collection and systematization of data will allow the calculation of the reference boundaries of the main blood parameters of lisopesse hybrids to assess the biochemical status of the beast by sex and age.
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按性别和年龄划分的狐胎杂种的生理生化特征
目的:研究杂交狐狸雌雄血液生化指标随年龄的变化。材料和方法。对杂交狐狸的雄性和雌性进行了研究。通过对比分析2月龄和5月龄杂交狐狸雌、雄2个年龄组(n = 30)血液生化参数变化的年龄动态。每天(上午)饲喂1次,在相同条件下,按动物的年龄和生理状况饲喂混合肉品。动物的血液被放入一个特殊的试管中,试管中有小腿外侧皮下静脉血栓的激活剂,直到早上喂食。以2000 rpm离心15分钟,从中提取血清。为了获得机体功能状态的信息,选择了能够可靠地反映代谢过程状态的生化试验,如碳水化合物、脂质蛋白:一般蛋白、白蛋白、丙氨酸氨基转移酶、天冬氨酸氨基转移酶、碱性磷酸酶、α-淀粉酶、葡萄糖、尿素、肌酐。动物在生长发育过程中表现出尿素、肌酐、总蛋白、白蛋白和葡萄糖等多项指标的升高。相反,ALT和AST活性随着年龄的增长而下降,在5月龄时达到最低值2。碱性磷酸酶浓度也随年龄的增长而降低。α淀粉酶水平在动物生长发育过程中无显著变化。杂交狐狸的蛋白质、碳水化合物代谢指标以及肾脏标志物和酶的特征都存在性别差异。因此,在动物的生长发育过程中,血液的生化成分会发生许多变化。数据的收集和系统化将允许计算lisopesse杂交种主要血液参数的参考边界,以评估按性别和年龄的兽的生化状态。
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