Evaluation of Lamination Micro Mixer for Micro Immunomagnetic Cell Sorter

H. Inokuchi, K. Nagae, Y. Suzuki, N. Kasagi, N. Shikazono
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引用次数: 1

Abstract

A split-and-recombine lamination mixer for mu-immunomagnetic cell sorting system has been developed for extracting stem cells from peripheral blood. Cell suspension is mixed with antibody-coated magnetic beads, and target cells are labeled with antigen-antibody reaction. In this report, the mixer performance is evaluated quantitatively by using human umbilical vein endothelial cells (HUVEC). The number of magnetic beads attached to the target cell is evaluated in two different arrangements of the mixer units
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微免疫磁性细胞分选机层压微混合器的评价
研制了一种用于多免疫细胞分选系统的分裂-重组层压混合器,用于提取外周血干细胞。将细胞悬液与抗体包被的磁珠混合,用抗原-抗体反应标记靶细胞。在本报告中,使用人脐静脉内皮细胞(HUVEC)定量评估混合器的性能。在混合单元的两种不同布置中评估附着在目标单元上的磁珠的数量
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