Pub Date : 2021-12-10DOI: 10.1109/iccss53909.2021.9721974
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{"title":"PDF Not Yet Available In IEEE Xplore","authors":"","doi":"10.1109/iccss53909.2021.9721974","DOIUrl":"https://doi.org/10.1109/iccss53909.2021.9721974","url":null,"abstract":"The document that should appear here is not currently available.","PeriodicalId":170356,"journal":{"name":"2006 International Conference on Microtechnologies in Medicine and Biology","volume":"51 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121542783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This paper describes a concept of the micro tweezers using comb-drive actuator which handles a minute material softly. The concrete purpose is holding DNA, a cell, etc. softly. We formulated the technique of detecting the mechanical characteristic of the comb-drive actuator widely used as a micromachined actuator by electric admittance measurement. By using this, it is detectable that the tweezers arm which is carrying out resonance vibration contacted the cell in solution. Since attenuation of the amplitude can be grasped by admittance change, the monitoring of the strength of handling may be able to be carried out. The resonance characteristic can be observed though the viscosity resistance becomes large in a solution and the amplitude becomes small compared with air. Using this concept we evaluated mechanical properties of a fabricated comb-drive actuator and confirmed its practical usefulness for characterization of soft-handling tweezers
{"title":"A concept of soft-handing tweezers using comb-drive actuator","authors":"K. Ayano, M. Takahashi, K. Suzuki, G. Hashiguchi","doi":"10.1109/MMB.2006.251545","DOIUrl":"https://doi.org/10.1109/MMB.2006.251545","url":null,"abstract":"This paper describes a concept of the micro tweezers using comb-drive actuator which handles a minute material softly. The concrete purpose is holding DNA, a cell, etc. softly. We formulated the technique of detecting the mechanical characteristic of the comb-drive actuator widely used as a micromachined actuator by electric admittance measurement. By using this, it is detectable that the tweezers arm which is carrying out resonance vibration contacted the cell in solution. Since attenuation of the amplitude can be grasped by admittance change, the monitoring of the strength of handling may be able to be carried out. The resonance characteristic can be observed though the viscosity resistance becomes large in a solution and the amplitude becomes small compared with air. Using this concept we evaluated mechanical properties of a fabricated comb-drive actuator and confirmed its practical usefulness for characterization of soft-handling tweezers","PeriodicalId":170356,"journal":{"name":"2006 International Conference on Microtechnologies in Medicine and Biology","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125336510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Y. Murayama, T. Yajima, T. Fukuda, S. Suzuki, Y. Hatakeyama, H. Sakuma, S. Takenoshita, C. Constantinou, S. Omata
In this study, a tactile mapping system was developed to obtain a two-dimensional elasticity distribution image in order to study the cause of hardening of a chronic hepatitis and liver cirrhosis. Tactile Mapping system utilized a micro tactile sensor which can measure a local elasticity of a very thin sample in micron scale. By comparing the tactile mapping image and azan stained image, it was indicated that the accumulation of the collagen might be the main cause of the liver hardening
{"title":"Topographic Elasticity Measurement for an Estimation of Hepatic Fibrosis Using Tactile Mapping System","authors":"Y. Murayama, T. Yajima, T. Fukuda, S. Suzuki, Y. Hatakeyama, H. Sakuma, S. Takenoshita, C. Constantinou, S. Omata","doi":"10.1109/MMB.2006.251554","DOIUrl":"https://doi.org/10.1109/MMB.2006.251554","url":null,"abstract":"In this study, a tactile mapping system was developed to obtain a two-dimensional elasticity distribution image in order to study the cause of hardening of a chronic hepatitis and liver cirrhosis. Tactile Mapping system utilized a micro tactile sensor which can measure a local elasticity of a very thin sample in micron scale. By comparing the tactile mapping image and azan stained image, it was indicated that the accumulation of the collagen might be the main cause of the liver hardening","PeriodicalId":170356,"journal":{"name":"2006 International Conference on Microtechnologies in Medicine and Biology","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125426558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Kimura, H. Sakai, S. Ostrovidov, T. Yamamoto, Y. Sakai, T. Fujii
We have developed and experimentally demonstrated a microbioreactor embedded with stirrer-type micropumps for on-chip perfusion of culture medium and optical fiber connectors for on-chip and real-time fluorescent detection. The cell culture chamber was separated two compartments by a polyester-made semi-permeable membrane, which can realize polarized cell culture condition and detection of fluorescent movement in-between the two-compartments. As an evaluation of the microbioreactor, it was measured that Rhodamine-123 movement by the activity of P-glycoprotein of a small intestine model cell of Caco-2. As a result, the change of fluorescent intensity of Rhodamine-123 from basolateral side to apical side of Caco-2 cells was successfully detected
{"title":"Two-Compartments Microbioreactor with Integrated Magnetic Stirrer Pump for Measurement of Transmembrane Transport of Caco-2 Cells","authors":"H. Kimura, H. Sakai, S. Ostrovidov, T. Yamamoto, Y. Sakai, T. Fujii","doi":"10.1109/MMB.2006.251504","DOIUrl":"https://doi.org/10.1109/MMB.2006.251504","url":null,"abstract":"We have developed and experimentally demonstrated a microbioreactor embedded with stirrer-type micropumps for on-chip perfusion of culture medium and optical fiber connectors for on-chip and real-time fluorescent detection. The cell culture chamber was separated two compartments by a polyester-made semi-permeable membrane, which can realize polarized cell culture condition and detection of fluorescent movement in-between the two-compartments. As an evaluation of the microbioreactor, it was measured that Rhodamine-123 movement by the activity of P-glycoprotein of a small intestine model cell of Caco-2. As a result, the change of fluorescent intensity of Rhodamine-123 from basolateral side to apical side of Caco-2 cells was successfully detected","PeriodicalId":170356,"journal":{"name":"2006 International Conference on Microtechnologies in Medicine and Biology","volume":"308 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114949338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P. Kim, Sang Eun Lee, Ho-Sup Jung, Hea-Yeon Lee, T. Kawai, H. Jeong, K. Suh
We present simple soft lithographic methods for patterning supported lipid bilayer (SLB) membranes onto a surface and inside microfluidic channels. Micropatterns of polyethylene glycol (PEG)-based polymers were fabricated on glass substrates by microcontact printing or capillary molding. The patterned PEG surfaces have shown 97plusmn0.5% reduction in lipid adsorption onto two dimensional surfaces and 95plusmn1.2% reduction inside microfluidic channels in comparison to glass control. Atomic force microscopy measurements indicated that the deposition of lipid vesicles led to the formation of SLB membranes by vesicle fusion due to hydrophilic interactions with the exposed substrate. Furthermore, the functionality of the patterned SLBs was tested by measuring the binding interactions between biotin (ligand)-labeled lipid bilayer and streptavidin (receptor). SLB arrays were fabricated with spatial resolution down to ~500 nm on flat substrate and ~1 mum inside microfluidic channels, respectively
{"title":"Supported lipid bilayers microarrays onto a surface and inside microfluidic channels","authors":"P. Kim, Sang Eun Lee, Ho-Sup Jung, Hea-Yeon Lee, T. Kawai, H. Jeong, K. Suh","doi":"10.1109/MMB.2006.251517","DOIUrl":"https://doi.org/10.1109/MMB.2006.251517","url":null,"abstract":"We present simple soft lithographic methods for patterning supported lipid bilayer (SLB) membranes onto a surface and inside microfluidic channels. Micropatterns of polyethylene glycol (PEG)-based polymers were fabricated on glass substrates by microcontact printing or capillary molding. The patterned PEG surfaces have shown 97plusmn0.5% reduction in lipid adsorption onto two dimensional surfaces and 95plusmn1.2% reduction inside microfluidic channels in comparison to glass control. Atomic force microscopy measurements indicated that the deposition of lipid vesicles led to the formation of SLB membranes by vesicle fusion due to hydrophilic interactions with the exposed substrate. Furthermore, the functionality of the patterned SLBs was tested by measuring the binding interactions between biotin (ligand)-labeled lipid bilayer and streptavidin (receptor). SLB arrays were fabricated with spatial resolution down to ~500 nm on flat substrate and ~1 mum inside microfluidic channels, respectively","PeriodicalId":170356,"journal":{"name":"2006 International Conference on Microtechnologies in Medicine and Biology","volume":"119 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117293816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. Gritsun, M. Litza, A. Hiller, A. Moser, U. Hofmann
Multisite microelectrode recording represents a suitable procedure to study microphysiology and network interactions in the central nervous system on a short time scale. This enables a deeper understanding of recent neurochemical studies to investigate changes in the neurotransmitter GABA caused by deep brain stimulation at the same position. We describe in the following a semichronic procedure to implant a miniature system bringing both stimulation and monitoring probes in close proximity in the caudate putamen of freely behaving rats. New multisite microelectrodes to replace existing microdialysis probes are built from spun wires, characterized and described. The design of a closed loop recording and stimulating system is discussed
{"title":"Semichronic, Collocated Deep Brain Stimulation and Multisite Recording in Rats","authors":"T. Gritsun, M. Litza, A. Hiller, A. Moser, U. Hofmann","doi":"10.1109/MMB.2006.251497","DOIUrl":"https://doi.org/10.1109/MMB.2006.251497","url":null,"abstract":"Multisite microelectrode recording represents a suitable procedure to study microphysiology and network interactions in the central nervous system on a short time scale. This enables a deeper understanding of recent neurochemical studies to investigate changes in the neurotransmitter GABA caused by deep brain stimulation at the same position. We describe in the following a semichronic procedure to implant a miniature system bringing both stimulation and monitoring probes in close proximity in the caudate putamen of freely behaving rats. New multisite microelectrodes to replace existing microdialysis probes are built from spun wires, characterized and described. The design of a closed loop recording and stimulating system is discussed","PeriodicalId":170356,"journal":{"name":"2006 International Conference on Microtechnologies in Medicine and Biology","volume":"125 24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128805351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This paper presents a micro-PIV measurement technique using confocal microscopy, "confocal micro-PIV", and its application to Poiseuille flow. We have evaluated the performance and measurement accuracy of the confocal micro-PIV system through the simple Poiseuille flow measurement. Using the confocal micro-PIV system, we can measure the velocity field in the region of 240times180 mum with the depth-of-field of 1.88 mum The steady pressure-driven flow in a capillary with the diameter of 100 mum was measured using the confocal micro-PIV technique. The measurement results were compared with the theoretical solution of the Poiseuille flow in order to validate the measurement accuracy. The most dominant factor of measurement uncertainty is the effect of the Brownian motion of tracer particles. The effect of Brownian motion can be eliminated successfully by means of ensemble averaging method. The confocal micro-PIV is an accurate and useful method for the measurement of steady microscale flow
{"title":"Validation of Confocal Micro-PIV Technique by Poiseuille Flow Measurement","authors":"H. Kinoshita, M. Oshima, S. Kaneda, T. Fujii","doi":"10.1109/MMB.2006.251495","DOIUrl":"https://doi.org/10.1109/MMB.2006.251495","url":null,"abstract":"This paper presents a micro-PIV measurement technique using confocal microscopy, \"confocal micro-PIV\", and its application to Poiseuille flow. We have evaluated the performance and measurement accuracy of the confocal micro-PIV system through the simple Poiseuille flow measurement. Using the confocal micro-PIV system, we can measure the velocity field in the region of 240times180 mum with the depth-of-field of 1.88 mum The steady pressure-driven flow in a capillary with the diameter of 100 mum was measured using the confocal micro-PIV technique. The measurement results were compared with the theoretical solution of the Poiseuille flow in order to validate the measurement accuracy. The most dominant factor of measurement uncertainty is the effect of the Brownian motion of tracer particles. The effect of Brownian motion can be eliminated successfully by means of ensemble averaging method. The confocal micro-PIV is an accurate and useful method for the measurement of steady microscale flow","PeriodicalId":170356,"journal":{"name":"2006 International Conference on Microtechnologies in Medicine and Biology","volume":"32 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126924612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F. Greve, L. Seemann, S. Bonneick, J. Lichtenberg, A. Hierlemann
A microchip-based approach for performing a complete and automated drug-screening assay on living cells is presented. Cells are trapped and immobilized in a small 0.5-mul-volume incubation chamber by means of orifice microstructures and are subsequently incubated with drug dilutions ranging over three orders of magnitude. The microsystem includes a perforated silicon chip embedded in a larger elastomer substrate, which features the microfluidic network and the incubation chamber. This article describes the modeling and the fabrication of the microchip components, immobilization of normal human dermal fibroblasts (NHDFs) and a screening experiment with cultured NHDFs, which have been exposed to a fluorescent cell tracker
{"title":"High-throughput cell-based screening system with on-chip dilution stage","authors":"F. Greve, L. Seemann, S. Bonneick, J. Lichtenberg, A. Hierlemann","doi":"10.1109/MMB.2006.251525","DOIUrl":"https://doi.org/10.1109/MMB.2006.251525","url":null,"abstract":"A microchip-based approach for performing a complete and automated drug-screening assay on living cells is presented. Cells are trapped and immobilized in a small 0.5-mul-volume incubation chamber by means of orifice microstructures and are subsequently incubated with drug dilutions ranging over three orders of magnitude. The microsystem includes a perforated silicon chip embedded in a larger elastomer substrate, which features the microfluidic network and the incubation chamber. This article describes the modeling and the fabrication of the microchip components, immobilization of normal human dermal fibroblasts (NHDFs) and a screening experiment with cultured NHDFs, which have been exposed to a fluorescent cell tracker","PeriodicalId":170356,"journal":{"name":"2006 International Conference on Microtechnologies in Medicine and Biology","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130678983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This paper presents a neural signal processing ASIC based on discrete wavelet transform (DWT). The recorded neural signals from 256 channels are analyzed by fast DWT algorithm with special ALUs, then, compressed by run-length encoders (RLE). The processed data are delivered through RF links and reconstructed in a host receiver. This design operates at 200 MHz clock with 2.5 V and was implemented with TSMC 0.25 mum technology. Recorded neural data test shows 1:89:3 (1.12%) compression rate and perfect in-band noise rejection
{"title":"Neural Signal Processing using Discrete Wavelet Transform for Neural Interfaces","authors":"J. Lee, D. Kipke","doi":"10.1109/MMB.2006.251519","DOIUrl":"https://doi.org/10.1109/MMB.2006.251519","url":null,"abstract":"This paper presents a neural signal processing ASIC based on discrete wavelet transform (DWT). The recorded neural signals from 256 channels are analyzed by fast DWT algorithm with special ALUs, then, compressed by run-length encoders (RLE). The processed data are delivered through RF links and reconstructed in a host receiver. This design operates at 200 MHz clock with 2.5 V and was implemented with TSMC 0.25 mum technology. Recorded neural data test shows 1:89:3 (1.12%) compression rate and perfect in-band noise rejection","PeriodicalId":170356,"journal":{"name":"2006 International Conference on Microtechnologies in Medicine and Biology","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124116447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Temperature-controlled on-chip capillary electrophoresis (CE) was applied to multi-temperature single-strand conformation polymorphism (SSCP) analysis for the detection of three lipoprotein lipase (LPL) gene mutations. In the SSCP analysis, five different temperature conditions (10, 15, 20, 25, and 30degC) were used to obtain an SSCP pattern. Differences between the electropherograms of Cy5-labeled PCR products of wild/wild homozygote and wild/mutant heterozygote were clearly detected for all four LPL mutations in at least one appropriate temperature condition. These results indicate that our SSCP system is applicable to high-throughput and accurate SSCP screening for locating unknown as well as known single nucleotide polymorphisms (SNPs)
{"title":"High-throughput Single-strand Conformation Polymorphism Analysis of an LPL Gene Mutation by Temperature-controlled On-chip Capillary Electrophoresis","authors":"K. Ono, M. Koike, T. Ohse, A. Takagi, Y. Ikeda","doi":"10.1109/MMB.2006.251527","DOIUrl":"https://doi.org/10.1109/MMB.2006.251527","url":null,"abstract":"Temperature-controlled on-chip capillary electrophoresis (CE) was applied to multi-temperature single-strand conformation polymorphism (SSCP) analysis for the detection of three lipoprotein lipase (LPL) gene mutations. In the SSCP analysis, five different temperature conditions (10, 15, 20, 25, and 30degC) were used to obtain an SSCP pattern. Differences between the electropherograms of Cy5-labeled PCR products of wild/wild homozygote and wild/mutant heterozygote were clearly detected for all four LPL mutations in at least one appropriate temperature condition. These results indicate that our SSCP system is applicable to high-throughput and accurate SSCP screening for locating unknown as well as known single nucleotide polymorphisms (SNPs)","PeriodicalId":170356,"journal":{"name":"2006 International Conference on Microtechnologies in Medicine and Biology","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124382741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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