Respon Pertumbuhan Protocorm Like Bodies (PLB) Anggrek Tebu (Grammatophyllum scriptum Blume) terhadap Modifikasi Media Murashige and Skoog (MS) secara In Vitro

Ratna Kusuma, Samsurianto Samsurianto, Wafif Azizah, Ervinda Yuliatin
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Abstract

Anggrek Tebu (Grammatophyllum scriptum) propagation can be improved optimally by adding plant growth regulators such as BAP and NAA through in vitro technique. Murashige and Skoog (MS) medium is an essential medium for plant tissue growth that needs another complex mineral and vitamin to develop the protocorm-like bodies (PBL) of Anggrek Tebu. Hence, this study aimed to gain the appropriate composition of modified MS by adding BAP and various coconut water concentrations and to evaluate their effect on PBL of Anggrek Tebu growth (budding time, number of buds, and leaves). The research was conducted using a completely randomized design with two factorials such as modified MS medium (MS (M1), ½ MS (M2), and ¼ MS (M3))and coconut water concentration (0 ml/L (C0), 100 ml/L (C1), 200 ml /L (C2), and 300 ml/L (C3)) with triplication. The result showed that the fastest budding time was remarkable growth from M2C1 offered the bud at three weeks after planting (WAP). In the number of budding, M3C2 showed excellent results and significantly increased the budding growth at 8.33 ± 0.88  in 10 WAP. Meanwhile, the highest leave number was M2C1 at 23.00 ± 3.60, followed by M2C2 and M3C2, respectively, at 20.67 ± 1.45 and 20.67 ± 1.33. This result indicated that modifying MS medium composition by adding the coconut water could assist the PBL of Anggrek Tebu growth in a specific concentration. 
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通过体外培养技术,添加BAP和NAA等植物生长调节剂可以最优地促进黄芪(Grammatophyllum scriptum)的繁殖。Murashige and Skoog (MS)培养基是植物组织生长必需的培养基,它需要另一种复杂的矿物质和维生素来发育黄芪原球茎样体(PBL)。因此,本研究旨在通过添加BAP和不同浓度的椰子水,获得合适的改性MS组成,并评价其对黄芪生长PBL(出芽时间、芽数和叶片)的影响。研究采用完全随机设计,采用改良MS培养基(MS (M1),½MS (M2)和¼MS (M3))和椰子水浓度(0 ml/L (C0), 100 ml/L (C1), 200 ml/L (C2)和300 ml/L (C3))两种因子进行三倍。结果表明,M2C1在播种后3周出芽,出芽时间最快。在出芽数量上,M3C2表现出优异的效果,在10 WAP下,M3C2的出芽生长率为8.33±0.88。休假数最多的是M2C1,为23.00±3.60,其次是M2C2和M3C2,分别为20.67±1.45和20.67±1.33。结果表明,在一定浓度下,通过添加椰子水来改变MS培养基的组成,可以促进黄颡鱼PBL的生长。
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