Behaviour of Human Umbilical Vein Endothelial Cells (HUVEC) Cultivated in Microfluidic channels

P. Mulder, G. Molema, S. Koster, H.J. van der Linden, E. Verpoorte
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引用次数: 4

Abstract

Our long-term goal is to develop advanced tools for cell studies and analysis based on microfluidic systems. In this paper, we report on endothelial cell cultivation in microchannels and 96-well tissue plates, and compare cell phenotype and cellular status in the two environments This was done under both pro-inflammatory conditions (cell stimulation in the presence of the cytokine, Tumour Necrosis Factor, TNFalpha) and normal (medium only) conditions. In addition, we considered the behaviour of cells in a pro-inflammatory environment in the presence of an anti-inflammatory drug
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人脐静脉内皮细胞(HUVEC)在微流体通道中培养的行为
我们的长期目标是开发基于微流体系统的细胞研究和分析的先进工具。在本文中,我们报道了内皮细胞在微通道和96孔组织板中的培养,并比较了两种环境下细胞表型和细胞状态。这是在促炎条件下(细胞因子,肿瘤坏死因子,TNFalpha存在下的细胞刺激)和正常(仅中等)条件下完成的。此外,我们考虑了细胞在抗炎药物存在的促炎环境中的行为
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