NUTRACEUTICAL POTENTIAL AND ANTIMICROBIAL ACTIVITY OF GANODERMA LUCIDUM (FR.) P. KARST

Abstract

The crude methanol (MeOH), n- hexane ( n- Hex), petroleum ether (PE), chloroform (CHCl 3 ) and Aqueous (Aq) extracts of Ganoderma lucidum (Fr.) P. Karst were screened to determine their bioactive constituents, antioxidant potential and antimicrobial activity. Among all the tested extracts, highest phenolics and tannins contents were observed in CHCl 3 (127.5 mg 100g -1 ) and PE fractions (78.52 mg 100g -1 ). The highest flavonoids moiety (84.9 mg 100g -1 ) and antioxidant activity (82.67%) was recorded in MeOH fractions. In vitro antibacterial and antifungal activities were evaluated by disc and well diffusion method. The maximum (72.14%) inhibitory zone was observed in CHCl 3 fraction with MIC value of 5.2mg ml -1 against the bacterial strain Staphylococcus sp. However E. coli was not susceptible to PE extract while Xanthomonas sp was resistible to n- Hex and PE extracts. Likewise CHCl 3 fraction showed significant activity against the fungal strain Penicillium sp having growth inhibitory zone of 68.42% with MIC value of 41.66 mg ml -1 . However no activity was observed for n- Hex against Penicillium sp and Aspergillus sp ., CHCl 3 against Paecilomyces sp and Trichoderma sp while PE ether against Aspergillus sp . The present findings suggest that notable antimicrobial potential of extracts might be due the presence of bioactive compounds.