Towards High Throughput Electroporation of Zebrafish Folicles

Abstract

This study presents a new approach for delivery of molecules into the cell Layers of Zebrafish Follicle which can be crucial for understanding of ovarian development and the treatment of ovarian diseases. Zebrafish follicles are used as a model of ovarian development. These follicles consists of an oocyte surrounded by two thin layers of cells called theca and granulosa cell. Electroporation is a non-invasive method widely used for transferring molecules into cells for various applications including stem cell based tissue construction and gene therapy. Despite broad advantages of electroporation, no reports have been released for application of drug delivery into Zebrafish follicle and Follicle cells. This paper is the first to discuss the advantage of electroporation for the delivery of biomolecules into the follicle and follicle cells. Herein we demonstrate and discuss the advantage of electroporation for the molecule delivery into follicle and follicle cells using Propidium Iodide (PI) and green fluorescence protein (GFP).