Identification of certain bioactive compounds with anthelmintic properties in Azadirachta indica and Clerodendrum viscosum

Asian-Australasian Journal of Bioscience and Biotechnology Pub Date : 2018-08-30 DOI:10.3329/aajbb.v3i2.64820

Md. Nuruzzaman Munsi, Ershaduzzaman, S. Akther, Md. Zakir Hassan, Md. Mamunur Rahman, M. Mondal

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Abstract

This study was undertaken to detect certain bioactive compounds with anthelmintic properties in Azadirachta indica and Clerodendrum viscosum by using high performance liquid chromatography (HPLC). From the HPLC analysis A. indica showed peak retention time which was similar to standard phenolic compounds including tannic acid (A. indica retention time 3.270 min, STD retention time 3.271 min) and pyrogallol (A. indica retention time 3.948 min, STD retention time 3.795 min). Benzoic acid (C. Viscosum retention time 6.092 min, STD retention time 6.067 min), tannic acid (C. Viscosum retention time 3.322 min, STD retention time 3.271 min) and quercetin (C. Viscosum retention time 4.967 min, STD retention time 4.222 min) was detected in leaf part of C. Viscosum. Most of these ingredients have well-known anthelmintic roles. Thus, it can be concluded that Azadirachta indica and Clerodendrum viscosum leaves contain bioactive compounds with anthelmintic properties. Asian Australas. J. Biosci. Biotechnol. 2018, 3 (2), 170-173

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印楝和粘枝中某些驱虫活性物质的鉴定

采用高效液相色谱法对印楝和粘枝中具有驱虫药作用的活性物质进行了检测。HPLC分析表明，黄花楸的保留时间与单宁酸(黄花楸保留时间3.270 min, STD保留时间3.271 min)和邻苯三酚(黄花楸保留时间3.948 min, STD保留时间3.795 min)等标准酚类化合物的保留时间相似。其中，甘露叶中检测到苯甲酸(甘露保留时间6.092 min, STD保留时间6.067 min)、单宁酸(甘露保留时间3.322 min, STD保留时间3.271 min)和槲皮素(甘露保留时间4.967 min, STD保留时间4.222 min)。这些成分中的大多数都有众所周知的驱虫作用。由此可见，印楝叶和粘枝叶均含有驱虫活性物质。亚洲Australas。j . Biosci。生物技术，2018,3 (2)，170-173

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Asian-Australasian Journal of Bioscience and Biotechnology

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