Pub Date : 2024-02-14DOI: 10.3329/aajbb.v9i1.71499
Mustafa Atasever
Abstract not available
无摘要
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Pub Date : 2023-11-17DOI: 10.3329/aajbb.v8i3.67823
Plabon Saha, Shiuly Akter, Mehedi Hasan, Abu Sina Md Tushar, Muhtarima Jannat, Md Abdul Haque Mozumder, Abdul Mukaddim Bin Moustainoor Rahman, Md Zahangir Alam, Md Rashidul Islam
Bacterial wilt of tobacco caused by Ralstonia solanacearum poses a significant threat to tobacco cultivation in Bangladesh. In this study, fourteen tobacco growing areas of Bangladesh were surveyed to assess the status of bacterial wilt disease in tobacco. The result revealed that the higher level of bacterial wilt incidence and severity was recorded in Bandarban followed by Naikhonchari, Lama, Rangpur, and Lalmonirhat and the lower level was in Chakaria, Kushtia, Meherpur, Chuadanga, and Jhenaidah. However, a moderate level of incidence and severity was recorded in Manikgonj, Khagrachori, and Tangail. After estimating the bacterial population in the soil of respective regions it was observed that the highest bacterial population was counted at 22.4 x 107 CFU/g soil in Chakaria and the lowest was counted at 4.3 x 107 CFU/g soil in Tangail. Among the seed samples, sample no BWT Seed 21 had the highest level of infection (93%), sample no BWT Seed 37 had a medium level (39.46%), and sample no BWT Seed 57 had the lowest level of infection (5.14%) with R. solanacearum as detected by plating tobacco seed samples by TTC agar medium. Biovar and races of R. solanacearum were identified through the sugar oxidation test and pathogenicity test and it was found that R. solanacearum isolates from all the fourteen growing areas were grouped into race 1 and biovar 1, biovar 2, biovar 3. Genetic diversity of R. solanacearum analysis was done by using REP and ERIC primers where both primers showed three clusters (I, II, and III) at 60% similarity. The results are very crucial for the development of sustainable management of bacterial wilt of tobacco and for future population analyses of R. solanacearum. Asian Australas. J. Biosci. Biotechnol. 2023, 8(3), 56-69
由 Ralstonia solanacearum 引起的烟草细菌枯萎病对孟加拉国的烟草种植构成了严重威胁。本研究调查了孟加拉国的 14 个烟草种植区，以评估烟草细菌性萎蔫病的状况。结果显示，班达尔班的细菌枯萎病发病率和严重程度较高，其次是奈孔查里、拉马、兰普尔和拉尔莫尼哈特；查卡里亚、库什蒂亚、梅赫普尔、丘阿丹加和杰奈达的发病率和严重程度较低。不过，马尼克贡杰、卡格拉乔里和坦盖尔的发病率和严重程度处于中等水平。对各地区土壤中的细菌数量进行估计后发现，查卡里亚的细菌数量最高，为 22.4 x 107 CFU/克土壤，而坦盖尔的细菌数量最低，为 4.3 x 107 CFU/克土壤。在种子样品中，BWT 种子 21 号样品的茄碱菌感染率最高（93%），BWT 种子 37 号样品的感染率中等（39.46%），BWT 种子 57 号样品的茄碱菌感染率最低（5.14%）。通过糖氧化试验和致病性试验确定了 R. solanacearum 的生物变种和种族，发现来自所有 14 个种植区的 R. solanacearum 分离物被归类为种族 1 和生物变种 1、生物变种 2、生物变种 3。使用 REP 和 ERIC 引物对茄碱菌的遗传多样性进行了分析，结果显示这两种引物有三个群集（I、II 和 III），相似度为 60%。 这些结果对于烟草细菌性枯萎病的可持续管理以及未来 R. solanacearum 的种群分析至关重要。Asian Australas.J. Biosci.Biotechnol.2023, 8(3), 56-69
{"title":"Bacterial wilt of tobacco in Bangladesh: a pilot study for assessment of the status, detection of seed-borne nature and genetic variation of its pathogen, Ralstonia solanacearum","authors":"Plabon Saha, Shiuly Akter, Mehedi Hasan, Abu Sina Md Tushar, Muhtarima Jannat, Md Abdul Haque Mozumder, Abdul Mukaddim Bin Moustainoor Rahman, Md Zahangir Alam, Md Rashidul Islam","doi":"10.3329/aajbb.v8i3.67823","DOIUrl":"https://doi.org/10.3329/aajbb.v8i3.67823","url":null,"abstract":"Bacterial wilt of tobacco caused by Ralstonia solanacearum poses a significant threat to tobacco cultivation in Bangladesh. In this study, fourteen tobacco growing areas of Bangladesh were surveyed to assess the status of bacterial wilt disease in tobacco. The result revealed that the higher level of bacterial wilt incidence and severity was recorded in Bandarban followed by Naikhonchari, Lama, Rangpur, and Lalmonirhat and the lower level was in Chakaria, Kushtia, Meherpur, Chuadanga, and Jhenaidah. However, a moderate level of incidence and severity was recorded in Manikgonj, Khagrachori, and Tangail. After estimating the bacterial population in the soil of respective regions it was observed that the highest bacterial population was counted at 22.4 x 107 CFU/g soil in Chakaria and the lowest was counted at 4.3 x 107 CFU/g soil in Tangail. Among the seed samples, sample no BWT Seed 21 had the highest level of infection (93%), sample no BWT Seed 37 had a medium level (39.46%), and sample no BWT Seed 57 had the lowest level of infection (5.14%) with R. solanacearum as detected by plating tobacco seed samples by TTC agar medium. Biovar and races of R. solanacearum were identified through the sugar oxidation test and pathogenicity test and it was found that R. solanacearum isolates from all the fourteen growing areas were grouped into race 1 and biovar 1, biovar 2, biovar 3. Genetic diversity of R. solanacearum analysis was done by using REP and ERIC primers where both primers showed three clusters (I, II, and III) at 60% similarity. The results are very crucial for the development of sustainable management of bacterial wilt of tobacco and for future population analyses of R. solanacearum. Asian Australas. J. Biosci. Biotechnol. 2023, 8(3), 56-69","PeriodicalId":184754,"journal":{"name":"Asian-Australasian Journal of Bioscience and Biotechnology","volume":"35 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139264135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-16DOI: 10.3329/aajbb.v8i3.68609
Surma Mohiudden Meem, K. Akter, Md Abdullah Al Mamun, S. Islam, F. S. Borsha, M. S. Abedin, Md Monwar Hossain
Cytochrome C oxidase subunit I (COI), known as DNA barcodes, can be employed for the identification of an unknown specimen at the species level. The present study was aimed at performing COI gene-based identification of butterflies using butterfly caterpillars. Consecutively, ten caterpillars of ten different butterfly species from the families Papilionidae, Nymphalidae, Pieridae, Lycaenidae, and Hesperiidae were taken from respective host plants and used to generate COI gene sequences that were about 650 bp long. After BLAST analysis, the sequenced gene revealed 96–100% similarity to the same species from different regions. Then, sequences were submitted to NCBI's GenBank and obtained ten accession numbers. In order to elucidate genetic diversity and evolutionary relationships among ten species of butterfly caterpillars, pairwise distance analysis and the construction of a phylogenetic tree were performed using MEGA10 and BioEdit software. In the analysis, the interspecific genetic divergence among the caterpillars of butterflies was between 0.101-0.164%. A phylogenetic tree was constructed with the assistance of the Neighbor-Joining (NJ) algorithm, which identified two major clades, A and B, as well as indicated that butterflies caterpillars shared a common ancestor. All the species are included in these two clades, except Catopsilia pomona. This species is under the family Pieridae, and the phylogenetic position of Pieridae compared to other butterfly families is ambiguous, necessitating additional research to resolve this issue. The study demonstrated the general applicability of DNA barcoding for rapidly and accurately distinguishing butterfly species, even when using larval stages, as in the present study. Furthermore, it may disclose a higher taxonomic hierarchy of butterfly families. Asian Australas. J. Biosci. Biotechnol. 2023, 8(3), 49-55
{"title":"DNA barcoding and phylogenetic relationships of ten butterfly caterpillars","authors":"Surma Mohiudden Meem, K. Akter, Md Abdullah Al Mamun, S. Islam, F. S. Borsha, M. S. Abedin, Md Monwar Hossain","doi":"10.3329/aajbb.v8i3.68609","DOIUrl":"https://doi.org/10.3329/aajbb.v8i3.68609","url":null,"abstract":"Cytochrome C oxidase subunit I (COI), known as DNA barcodes, can be employed for the identification of an unknown specimen at the species level. The present study was aimed at performing COI gene-based identification of butterflies using butterfly caterpillars. Consecutively, ten caterpillars of ten different butterfly species from the families Papilionidae, Nymphalidae, Pieridae, Lycaenidae, and Hesperiidae were taken from respective host plants and used to generate COI gene sequences that were about 650 bp long. After BLAST analysis, the sequenced gene revealed 96–100% similarity to the same species from different regions. Then, sequences were submitted to NCBI's GenBank and obtained ten accession numbers. In order to elucidate genetic diversity and evolutionary relationships among ten species of butterfly caterpillars, pairwise distance analysis and the construction of a phylogenetic tree were performed using MEGA10 and BioEdit software. In the analysis, the interspecific genetic divergence among the caterpillars of butterflies was between 0.101-0.164%. A phylogenetic tree was constructed with the assistance of the Neighbor-Joining (NJ) algorithm, which identified two major clades, A and B, as well as indicated that butterflies caterpillars shared a common ancestor. All the species are included in these two clades, except Catopsilia pomona. This species is under the family Pieridae, and the phylogenetic position of Pieridae compared to other butterfly families is ambiguous, necessitating additional research to resolve this issue. The study demonstrated the general applicability of DNA barcoding for rapidly and accurately distinguishing butterfly species, even when using larval stages, as in the present study. Furthermore, it may disclose a higher taxonomic hierarchy of butterfly families. Asian Australas. J. Biosci. Biotechnol. 2023, 8(3), 49-55","PeriodicalId":184754,"journal":{"name":"Asian-Australasian Journal of Bioscience and Biotechnology","volume":"81 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139269994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The objectives of this study were to determine the farmers’ socio-economic characteristics, livestock population dynamics, and breeding practices followed by the farmers. Out of 1487 farmers, a major proportion (53.12%) of farmers was middle-aged. Most of the farmers (71.22%) were associated with agricultural activities and had low income per month (52.32%). Livestock keeping patterns of farmers were cattle, goats, buffalo, and combinations of these animals. Female animals were inferred to be more prevalent in different districts (cow 85.08%, doe 81.29%) than males (bull 14.92%, buck 18.71%). In case of cattle and goats, the highest number of farmers maintained a medium size herd, whereas buffalo farmers had a large herd size. In terms of breeding practices, the majority of the farmers (75.55%) performed artificial insemination (AI) in cattle and found it convenient to service their cows. When considering buffalo, and goat, the majority of the farmers (94.12% and 98.50%, respectively) practiced natural services. The findings also demonstrated that the majority of the farmers (73.8% and 82%, respectively) raised crossbreed cattle and buffalo, while just a tiny portion (26.18% and 18%, respectively) raised indigenous breeds. However, this situation was vice versa in case of goat. For choice of the breed according to the farmers, 39.17% of farmers preferred Black Bengal goat, 60.83% preferred cross breed. This study represents a comprehensive overview of livestock status and existing breeding practices employed by the farmers in different districts of Bangladesh, which may be utilized to implement relevant livestock improvement programs in Bangladesh. Asian Australas. J. Biosci. Biotechnol. 2023, 8(3), 38-48
{"title":"Population distribution and breeding practices of livestock in different districts of Bangladesh","authors":"Mam Yahia Khandoker, Md Younus Ali, Tasmina Akter, Mst Mahomudha Akhtar, Mst Kamrunnahar Kona, Nusrat Jahan Meki, Marzia Rahman Sompa, Israt Jahan Meem","doi":"10.3329/aajbb.v8i3.67698","DOIUrl":"https://doi.org/10.3329/aajbb.v8i3.67698","url":null,"abstract":"The objectives of this study were to determine the farmers’ socio-economic characteristics, livestock population dynamics, and breeding practices followed by the farmers. Out of 1487 farmers, a major proportion (53.12%) of farmers was middle-aged. Most of the farmers (71.22%) were associated with agricultural activities and had low income per month (52.32%). Livestock keeping patterns of farmers were cattle, goats, buffalo, and combinations of these animals. Female animals were inferred to be more prevalent in different districts (cow 85.08%, doe 81.29%) than males (bull 14.92%, buck 18.71%). In case of cattle and goats, the highest number of farmers maintained a medium size herd, whereas buffalo farmers had a large herd size. In terms of breeding practices, the majority of the farmers (75.55%) performed artificial insemination (AI) in cattle and found it convenient to service their cows. When considering buffalo, and goat, the majority of the farmers (94.12% and 98.50%, respectively) practiced natural services. The findings also demonstrated that the majority of the farmers (73.8% and 82%, respectively) raised crossbreed cattle and buffalo, while just a tiny portion (26.18% and 18%, respectively) raised indigenous breeds. However, this situation was vice versa in case of goat. For choice of the breed according to the farmers, 39.17% of farmers preferred Black Bengal goat, 60.83% preferred cross breed. This study represents a comprehensive overview of livestock status and existing breeding practices employed by the farmers in different districts of Bangladesh, which may be utilized to implement relevant livestock improvement programs in Bangladesh. Asian Australas. J. Biosci. Biotechnol. 2023, 8(3), 38-48","PeriodicalId":184754,"journal":{"name":"Asian-Australasian Journal of Bioscience and Biotechnology","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139338440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-29DOI: 10.3329/aajbb.v8i2.68109
Rozina Khanom, Md. Safiqul Islam, Mizanur Rahman, Mohammad Dalower, H. Prodhan
The consumers are very much concerned about food safety. To ensure safe food for the consumers, it is essential to follow the pre harvest interval (PHI) of pesticides. Therefore, in order to ascertain the pre-harvest interval of fenitrothion and diazinon in hyacinth bean in the environmental conditions of Bangladesh, this study was started. The chosen organophosphorus insecticides (fenitrothion and diazinon) were sprayed with the recommended dose (1.5 ml/L of water) in a field trial at the Entomology Division of BARI, Gazipur. At 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, and 12 days after spray (DAS), samples were taken. The Gas chromatography (GC) with a Flame Thermionic Detector (FTD) was used to quantify the residues after the samples were prepared using a modified Quick, Easy, Cheap, Effective, Rugged and Safe (QuEChERS) extraction method. Fenitrothion residue was found up to 8 DAS, and at 7 DAS (0.018 mg/Kg), the level of residue was above the MRL. At 8 DAS, the fenitrothion residue was 0.007 mg/kg, which was below MRL. Pre-harvest interval (PHI) for fenitrothion was determined at 8 DAS, and no residue was found after 9 DAS. Diazinon residue was found up to 9 DAS, with the level of residue above the MRL at 8 DAS (0.016 mg/Kg). At 9 DAS, the diazinon residue was 0.007 mg/kg, which was below MRL. Pre-harvest interval (PHI) was determined for diazinon at 9 DAS, and as of 10 DAS, no residue was found. Asian Australas. J. Biosci. Biotechnol. 2023, 8 (2), 30-37
{"title":"Degradation of selected organophosphorus pesticide residues and their pre harvest interval determination in hyacinth bean grown commercially in Bangladesh","authors":"Rozina Khanom, Md. Safiqul Islam, Mizanur Rahman, Mohammad Dalower, H. Prodhan","doi":"10.3329/aajbb.v8i2.68109","DOIUrl":"https://doi.org/10.3329/aajbb.v8i2.68109","url":null,"abstract":"The consumers are very much concerned about food safety. To ensure safe food for the consumers, it is essential to follow the pre harvest interval (PHI) of pesticides. Therefore, in order to ascertain the pre-harvest interval of fenitrothion and diazinon in hyacinth bean in the environmental conditions of Bangladesh, this study was started. The chosen organophosphorus insecticides (fenitrothion and diazinon) were sprayed with the recommended dose (1.5 ml/L of water) in a field trial at the Entomology Division of BARI, Gazipur. At 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, and 12 days after spray (DAS), samples were taken. The Gas chromatography (GC) with a Flame Thermionic Detector (FTD) was used to quantify the residues after the samples were prepared using a modified Quick, Easy, Cheap, Effective, Rugged and Safe (QuEChERS) extraction method. Fenitrothion residue was found up to 8 DAS, and at 7 DAS (0.018 mg/Kg), the level of residue was above the MRL. At 8 DAS, the fenitrothion residue was 0.007 mg/kg, which was below MRL. Pre-harvest interval (PHI) for fenitrothion was determined at 8 DAS, and no residue was found after 9 DAS. Diazinon residue was found up to 9 DAS, with the level of residue above the MRL at 8 DAS (0.016 mg/Kg). At 9 DAS, the diazinon residue was 0.007 mg/kg, which was below MRL. Pre-harvest interval (PHI) was determined for diazinon at 9 DAS, and as of 10 DAS, no residue was found.\u0000Asian Australas. J. Biosci. Biotechnol. 2023, 8 (2), 30-37","PeriodicalId":184754,"journal":{"name":"Asian-Australasian Journal of Bioscience and Biotechnology","volume":"163 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123135250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-26DOI: 10.3329/aajbb.v8i2.66927
Jiraporn Yansombat, S. Samosornsuk, Chollanant Khattiyawech, Panarat Hematulin, T. Pharamat, S. Kabir, W. Samosornsuk
Fungal endophyte is a fungal that lives in plant organism as mutualism association. The role of fungal endophyte is a growth promoter or/and microbial pathogen inhibitor. This study investigated antifungal activity of Colletotrichum truncatum E10, an endophytic fungus derived from Musa acuminata (AAA group), against 7 isolates of Aspergillus obtained from lower respiratory samples of COVID-19 patients. In addition, IAA production of this strain was also observed. All isolates of Aspergillus were identified using MALDI-TOF MS. The fungal endophyte, C. truncatum E10, was screened for IAA induction with and without 0.1, 2 and 8 mg/mL of L-tryptophan based on colorimetric method using Salkowski reagent which produced pinkish to reddish solution indicating the presence of IAA. Antagonist activity was based on dual culture assay measured in colony growth inhibition (CGI). C. truncatum E10 produced the highest IAA concentration of 112.81±0.12 µg/mL when 8 mg/mL of L-tryptophan added. The strong antagonist activities were shown by C. truncatum E10 against 5 Aspergillus isolates including 2 A. fumigatus: sp442/6 (CGI=57.83±5.11%) and sp269/11 (CGI=53.01±8.52%), 1 A. niger sp26/7 (CGI=57.83±15.33%) and 2 A. flavus: sp26/7 (CGI=56.63±13.63%) and sp36/7 (CGI=57.23±0.85%), whereas the colony growth inhibition (CGI) of other 2 isolates including A. fumigatus sp567/6 and A. flavus sp269/11 were less than 50%. In this study, C. truncatum E10 produced substances that inhibited human fungal pathogen including A. fumigatus, A. flavus and A. niger. Moreover, it can produce IAA activity. Further investigations are being conducted to expand the plant growth promotion effects and determine IAA biosynthesis pathway. For antifungal activity, the bioactive metabolites produced by this endophytic fungal isolate should be characterized to specify the effective compounds. Asian Australas. J. Biosci. Biotechnol. 2023, 8 (2), 23-29
{"title":"Colletotrichum truncatum, an endophytic fungus derived from Musa acuminata (AAA group): antifungal activity against Aspergillus isolated from COVID-19 patients and indole-3-acetic acid (IAA) production","authors":"Jiraporn Yansombat, S. Samosornsuk, Chollanant Khattiyawech, Panarat Hematulin, T. Pharamat, S. Kabir, W. Samosornsuk","doi":"10.3329/aajbb.v8i2.66927","DOIUrl":"https://doi.org/10.3329/aajbb.v8i2.66927","url":null,"abstract":"Fungal endophyte is a fungal that lives in plant organism as mutualism association. The role of fungal endophyte is a growth promoter or/and microbial pathogen inhibitor. This study investigated antifungal activity of Colletotrichum truncatum E10, an endophytic fungus derived from Musa acuminata (AAA group), against 7 isolates of Aspergillus obtained from lower respiratory samples of COVID-19 patients. In addition, IAA production of this strain was also observed. All isolates of Aspergillus were identified using MALDI-TOF MS. The fungal endophyte, C. truncatum E10, was screened for IAA induction with and without 0.1, 2 and 8 mg/mL of L-tryptophan based on colorimetric method using Salkowski reagent which produced pinkish to reddish solution indicating the presence of IAA. Antagonist activity was based on dual culture assay measured in colony growth inhibition (CGI). C. truncatum E10 produced the highest IAA concentration of 112.81±0.12 µg/mL when 8 mg/mL of L-tryptophan added. The strong antagonist activities were shown by C. truncatum E10 against 5 Aspergillus isolates including 2 A. fumigatus: sp442/6 (CGI=57.83±5.11%) and sp269/11 (CGI=53.01±8.52%), 1 A. niger sp26/7 (CGI=57.83±15.33%) and 2 A. flavus: sp26/7 (CGI=56.63±13.63%) and sp36/7 (CGI=57.23±0.85%), whereas the colony growth inhibition (CGI) of other 2 isolates including A. fumigatus sp567/6 and A. flavus sp269/11 were less than 50%. In this study, C. truncatum E10 produced substances that inhibited human fungal pathogen including A. fumigatus, A. flavus and A. niger. Moreover, it can produce IAA activity. Further investigations are being conducted to expand the plant growth promotion effects and determine IAA biosynthesis pathway. For antifungal activity, the bioactive metabolites produced by this endophytic fungal isolate should be characterized to specify the effective compounds.\u0000Asian Australas. J. Biosci. Biotechnol. 2023, 8 (2), 23-29","PeriodicalId":184754,"journal":{"name":"Asian-Australasian Journal of Bioscience and Biotechnology","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116967793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-30DOI: 10.3329/aajbb.v8i2.65667
Jhulan Rani Baishnab, ASM. Mahbub, M. Y. Miah
An experiment was carried out with 80 straight run broiler chicks (cobb-500) reared on rice husk and sawdust to know the production efficiency at 5 weeks during summer. A total 80 straight run broiler chicks (cobb-500) were divided into 4 groups and were assigned to 2 treatments i.e. T1 and T2 in which T1 was used rice husk and T2 was used sawdust. Number of birds per replication were 10. Live weight of birds reared on rice husk and sawdust differed significantly at 5th weeks of age (P<0.05). Feed consumption of broiler was significantly higher on sawdust litter than rice husk. Feed conversion ratio and survivability percentages of broiler were statistically non-significant (P>0.05) between the sawdust and rice husk. The cost of litter per bird and cost of litter per kg live weight gain were 14.07% and 20.10% higher in rice husk than sawdust respectively. It is evident from the study that sawdust was more economic than rice husk. Considering all the facts and findings of the study, it can be conducted that live weight and feed consumption of broiler was better in sawdust litter materials than rice husk. Sawdust was also cheaper than rice husk. Therefore, using sawdust as a litter material can lead to better production efficiency and cost savings for broiler farmers during summer. Asian Australas. J. Biosci. Biotechnol. 2023, 8 (2), 17-22
{"title":"Performance of broiler using rice husk and sawdust as litter materials during summer","authors":"Jhulan Rani Baishnab, ASM. Mahbub, M. Y. Miah","doi":"10.3329/aajbb.v8i2.65667","DOIUrl":"https://doi.org/10.3329/aajbb.v8i2.65667","url":null,"abstract":"An experiment was carried out with 80 straight run broiler chicks (cobb-500) reared on rice husk and sawdust to know the production efficiency at 5 weeks during summer. A total 80 straight run broiler chicks (cobb-500) were divided into 4 groups and were assigned to 2 treatments i.e. T1 and T2 in which T1 was used rice husk and T2 was used sawdust. Number of birds per replication were 10. Live weight of birds reared on rice husk and sawdust differed significantly at 5th weeks of age (P<0.05). Feed consumption of broiler was significantly higher on sawdust litter than rice husk. Feed conversion ratio and survivability percentages of broiler were statistically non-significant (P>0.05) between the sawdust and rice husk. The cost of litter per bird and cost of litter per kg live weight gain were 14.07% and 20.10% higher in rice husk than sawdust respectively. It is evident from the study that sawdust was more economic than rice husk. Considering all the facts and findings of the study, it can be conducted that live weight and feed consumption of broiler was better in sawdust litter materials than rice husk. Sawdust was also cheaper than rice husk. Therefore, using sawdust as a litter material can lead to better production efficiency and cost savings for broiler farmers during summer.\u0000Asian Australas. J. Biosci. Biotechnol. 2023, 8 (2), 17-22","PeriodicalId":184754,"journal":{"name":"Asian-Australasian Journal of Bioscience and Biotechnology","volume":"70 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123113004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-30DOI: 10.3329/aajbb.v8i1.65830
M. Alam, Deeder Sultana, S. Rahman, M. Prodhan
The farmers of our country mostly depend on chemical pesticides for crop protection due to the scarcity of competent pesticide alternatives and enough knowledge of safe pest management. As a result, there is a great possibility to remain residues in the harvested crops. Keeping this view, this study was conducted to analyze organophosphorus pesticide residues in yard long bean collected from five local markets of Mymensingh district of Bangladesh. The collected samples were carried to the Pesticide Analytical Laboratory, Entomology Division of Bangladesh Agricultural Research Institute (BARI), Gazipur on the same sampling day. The collected samples were analyzed using Quick, Easy, Cheap, Effective, Rugged and Safe (QuEChERS) extraction technique and Gas Chromatography (GC) coupled with Flame Thermionic Detector (FTD). Among the 30 samples of yard long bean, 3 samples (10% of the total samples) contained residue of quinalphos, of which 2 samples contained quinalphos residue above MRL. This study reflects the overall scenario of pesticide residue remain in the harvested yard long bean collected from local markets of Mymensingh city. Therefore, monitoring of pesticide residues in vegetables should be strengthened. Asian Australas. J. Biosci. Biotechnol. 2023, 8 (1), 8-16
{"title":"Monitoring of pesticide residues in yard long bean collected from Mymensingh district of Bangladesh","authors":"M. Alam, Deeder Sultana, S. Rahman, M. Prodhan","doi":"10.3329/aajbb.v8i1.65830","DOIUrl":"https://doi.org/10.3329/aajbb.v8i1.65830","url":null,"abstract":"The farmers of our country mostly depend on chemical pesticides for crop protection due to the scarcity of competent pesticide alternatives and enough knowledge of safe pest management. As a result, there is a great possibility to remain residues in the harvested crops. Keeping this view, this study was conducted to analyze organophosphorus pesticide residues in yard long bean collected from five local markets of Mymensingh district of Bangladesh. The collected samples were carried to the Pesticide Analytical Laboratory, Entomology Division of Bangladesh Agricultural Research Institute (BARI), Gazipur on the same sampling day. The collected samples were analyzed using Quick, Easy, Cheap, Effective, Rugged and Safe (QuEChERS) extraction technique and Gas Chromatography (GC) coupled with Flame Thermionic Detector (FTD). Among the 30 samples of yard long bean, 3 samples (10% of the total samples) contained residue of quinalphos, of which 2 samples contained quinalphos residue above MRL. This study reflects the overall scenario of pesticide residue remain in the harvested yard long bean collected from local markets of Mymensingh city. Therefore, monitoring of pesticide residues in vegetables should be strengthened.\u0000Asian Australas. J. Biosci. Biotechnol. 2023, 8 (1), 8-16","PeriodicalId":184754,"journal":{"name":"Asian-Australasian Journal of Bioscience and Biotechnology","volume":"34 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121992951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-30DOI: 10.3329/aajbb.v8i1.65782
M. Asaduzzaman, M. Akter, Ashikur Rahman, M. Moniruzzaman, A. Rahman, Md Saiful Islam
The experiment was conducted to develop the chocolate-flavored soy dahi and find the accepted level of soymilk that should be added to milk to produce chocolate-flavored dahi. Therefore, four distinct kinds of dahi designated T0 (control, 100% cow milk), T1 (75% cow milk and 25% soy milk) and T2 (50% cow milk and 50% soy milk) and T3 (25% cow milk and 75% soy milk) were prepared by partially substituting cow milk to soy milk. Each variety also contained 1.5% cocoa powder. A panel of professional judges assessed the sensory (organoleptic) properties of the produced samples. The physical study's findings revealed that the overall organoleptic (i.e., smell and taste, body and texture, color and appearance) scores of dahi of kinds T0, T1, T2, and T3 varied significantly (P<0.01) from one another. According to the findings of the chemical test, the soy milk addition considerably lowered the amount of fat, acidity, total solids, and minerals while dramatically increasing the amount of moisture and protein (P<0.05). The growth of lactic acid bacteria is considerably better, however, it gradually reduces with the higher Addition of soy milk to cow milk. Based on the findings, it was concluded that soy milk could substitute milk by 25%, producing dahi that may be almost as good as that made only with milk and cocoa powder. Asian Australas. J. Biosci. Biotechnol. 2023, 8 (1), 1-7
{"title":"Development of flavored dahi by incorporating soymilk and cocoa powder","authors":"M. Asaduzzaman, M. Akter, Ashikur Rahman, M. Moniruzzaman, A. Rahman, Md Saiful Islam","doi":"10.3329/aajbb.v8i1.65782","DOIUrl":"https://doi.org/10.3329/aajbb.v8i1.65782","url":null,"abstract":"The experiment was conducted to develop the chocolate-flavored soy dahi and find the accepted level of soymilk that should be added to milk to produce chocolate-flavored dahi. Therefore, four distinct kinds of dahi designated T0 (control, 100% cow milk), T1 (75% cow milk and 25% soy milk) and T2 (50% cow milk and 50% soy milk) and T3 (25% cow milk and 75% soy milk) were prepared by partially substituting cow milk to soy milk. Each variety also contained 1.5% cocoa powder. A panel of professional judges assessed the sensory (organoleptic) properties of the produced samples. The physical study's findings revealed that the overall organoleptic (i.e., smell and taste, body and texture, color and appearance) scores of dahi of kinds T0, T1, T2, and T3 varied significantly (P<0.01) from one another. According to the findings of the chemical test, the soy milk addition considerably lowered the amount of fat, acidity, total solids, and minerals while dramatically increasing the amount of moisture and protein (P<0.05). The growth of lactic acid bacteria is considerably better, however, it gradually reduces with the higher Addition of soy milk to cow milk. Based on the findings, it was concluded that soy milk could substitute milk by 25%, producing dahi that may be almost as good as that made only with milk and cocoa powder.\u0000Asian Australas. J. Biosci. Biotechnol. 2023, 8 (1), 1-7","PeriodicalId":184754,"journal":{"name":"Asian-Australasian Journal of Bioscience and Biotechnology","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123324991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-31DOI: 10.3329/aajbb.v7i3.63651
Nuzat Tasnim, Md Nasrul Millat, Sadia Sultana, S. Rahman, M. Prodhan
Pesticide residues in different crops, including food grains and vegetables are burning issues in Bangladesh. Therefore, the present study was conducted to analyze organophosphorus pesticide residues in three popular vegetables (cauliflower, hyacinth bean and yard long bean) collected from five local markets of Narsingdi district of Bangladesh. The collected vegetable samples were extracted and cleaned up using Quick, Easy, Cheap, Effective, Rugged and Safe (QuEChERS) extraction technique and analyzed by Gas Chromatography (GC) equipped with Flame Thermionic Detector (FTD). A total of 45 vegetable samples were analyzed. Among the 15 analyzed samples of hyacinth bean, three samples (20 % of the total samples) contained pesticide residues above the EU-MRL and the remaining samples did not contain any detectable residues of the selected organophosphorus pesticides. In case of cauliflower, out of 15 analyzed samples, two samples (13% of the total number of samples) contained pesticide residues above the EU-MRL. In case of yard long bean, of the 15 analyzed samples, four samples (27% of the total number of samples) contained pesticide residues above the EU-MRLs. This study reveals the present status of pesticide residue contamination in the selected vegetables. Asian Australas. J. Biosci. Biotechnol. 2022, 7 (3), 114-121
{"title":"Analysis of organophosphorus pesticide residues in selected vegetables purchased from Narsingdi district of Bangladesh using QuEChERS Extraction","authors":"Nuzat Tasnim, Md Nasrul Millat, Sadia Sultana, S. Rahman, M. Prodhan","doi":"10.3329/aajbb.v7i3.63651","DOIUrl":"https://doi.org/10.3329/aajbb.v7i3.63651","url":null,"abstract":"Pesticide residues in different crops, including food grains and vegetables are burning issues in Bangladesh. Therefore, the present study was conducted to analyze organophosphorus pesticide residues in three popular vegetables (cauliflower, hyacinth bean and yard long bean) collected from five local markets of Narsingdi district of Bangladesh. The collected vegetable samples were extracted and cleaned up using Quick, Easy, Cheap, Effective, Rugged and Safe (QuEChERS) extraction technique and analyzed by Gas Chromatography (GC) equipped with Flame Thermionic Detector (FTD). A total of 45 vegetable samples were analyzed. Among the 15 analyzed samples of hyacinth bean, three samples (20 % of the total samples) contained pesticide residues above the EU-MRL and the remaining samples did not contain any detectable residues of the selected organophosphorus pesticides. In case of cauliflower, out of 15 analyzed samples, two samples (13% of the total number of samples) contained pesticide residues above the EU-MRL. In case of yard long bean, of the 15 analyzed samples, four samples (27% of the total number of samples) contained pesticide residues above the EU-MRLs. This study reveals the present status of pesticide residue contamination in the selected vegetables.\u0000Asian Australas. J. Biosci. Biotechnol. 2022, 7 (3), 114-121","PeriodicalId":184754,"journal":{"name":"Asian-Australasian Journal of Bioscience and Biotechnology","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115760848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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