ISOLATION AND SCREENING OF INDIGENOUS MICROORGANISMS CAPABLE OF DEGRADING CELLULOSE TO TREAT HEMP HURD IN THANH HOA PROVINCE

Abstract

Hemp Hurd has not yet found an optimal treatment method, therefore, in this paper, we isolated and screened native microorganisms with high cellulose-degradation capability for potentially effective treatment. Native microorganisms were isolated on Gauze I and Hans, media then CMC-as activity was determined. Next, the species names of screening microorganisms were determined by total DNA isolation, PCR and Sequencing. The results showed that 02 bacterial strains and 06 actinomycete strains were selected from 32 isolates. Then, we optimized the growing conditions of the screened microorganisms. Among actinomycetes strains PU1.1, PU2.1 gave the highest weight loss results, therefore they were selected for further study. Our results showed that TG2.1 and CM4.1 are Bacillus subtilis. PU1.1 is Streptomyces lilacs, while PU1.2 is Streptomyces Misiones. The selected microorganisms belong to risk group level 1 (safety group). Thus, these strains can be used for further experiments in the research and production of microorganism-derived bio-products to treat hemp hurd in Thanh Hoa province.