Isolation of Starch Degrading Bacteria From Rhizospheric Soil of Mymensingh, Bangladesh

J. Mawa, MF Momo, R. Sultana, T. Zerin
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Abstract

Starch degrading bacteria are vital for various industries like food, fermentation, textile, and paper. The aim of this study is to isolate and characterize bacteria able to degrade starch from the rhizosphere of soil located in Mymensingh, Bangladesh. Collected soil sample was serially diluted in sterilized peptone water, poured on sterilized starch agar plates and incubated at 37ºC for 24 hours. The representative colonies showing different morphology was randomly picked up using the streaking method on nutrient agar media. A total of 8 bacterial colonies were isolated and labelled as MSH 01, MSH 02, MSH 03, MSH 04, MSH 05, MSH 06, MSH 07, and MSH 08. Biochemical characteristics showed that all the isolates belonged to the genera Bacillus. Among those, MSH 06 showed the highest starch degrading index (SDI) followed by MSH 02 and MSH 05. Surveillance to identify microbial species with enhanced starch hydrolyzing potential might be helpful in biotechnology industries. Stamford Journal of Microbiology, Vol.12 (1) 2022: 21-24
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从孟加拉国迈门辛格根际土壤中分离淀粉降解细菌
淀粉降解细菌对食品、发酵、纺织和造纸等行业至关重要。本研究的目的是分离和表征能够从位于孟加拉国Mymensingh的土壤根际降解淀粉的细菌。采集的土壤样品用灭菌的蛋白胨水依次稀释,倒在灭菌的淀粉琼脂板上,37℃孵育24小时。在营养琼脂培养基上随机取有代表性的不同形态菌落。共分离出8个菌落,分别标记为MSH 01、MSH 02、MSH 03、MSH 04、MSH 05、MSH 06、MSH 07和MSH 08。生化特征表明,所有分离株均属于芽孢杆菌属。其中淀粉降解指数(SDI)最高的是MSH 06,其次是MSH 02和MSH 05。监测识别具有增强淀粉水解潜力的微生物物种可能有助于生物技术工业。微生物学报,Vol.12 (1) 2022: 21-24
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