Minimal Path based Particle Tracking in Low SNR Fluorescence Microscopy Images

Sheng Lu, Tong Chen, Fan Yang, Chenglei Peng, S. Du, Yang Li
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引用次数: 5

Abstract

Single Particle Tracking (SPT) in fluorescence microscopy image is of great importance in the field of computational biology. Automatic or slightly interactive tracking algorithms are essential for the motional analysis of micro particles. Even with prior knowledge, conventional methods may fail when the signal-to-noise ratio (SNR) is too low because they highly depend on the quality of the image and the results of detection. To reliably track particles in the low SNR images, we proposed a novel method based on minimal path theory and attempted to extract complete trajectories between two points. Our method was evaluated on several simulated image sequences and showed its accuracy and robustness in the task of particle tracking.
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低信噪比荧光显微图像中基于最小路径的粒子跟踪
荧光显微镜图像中的单粒子跟踪(SPT)在计算生物学领域具有重要意义。自动或微交互跟踪算法对于微粒子的运动分析是必不可少的。即使有先验知识,当信噪比(SNR)过低时,传统方法也可能失败,因为它们高度依赖于图像的质量和检测结果。为了在低信噪比图像中可靠地跟踪粒子,我们提出了一种基于最小路径理论的新方法,并尝试提取两点之间的完整轨迹。在多个模拟图像序列上对该方法进行了验证,证明了该方法在粒子跟踪任务中的准确性和鲁棒性。
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