Structure and function of aconitase enzyme in TCA by estimating optical rotation of glucose and determining the relationship between cell density and absorbance

Abstract

Enzyme aconitase have a great value in TCA path, this enzyme use to convert pyruvate and acetyl co A in to citrate and cis aconitase ( a six carbon molecule). This study was designed to find the tertiary structure of aconitase with and without ligand by using RSBC. The TCA cycle start with the pyruvate that is end product of glycolysis cycle. This study also focused on the optical rotation of glucose molecule before its breakdown start naturally through glycolysis and absorbance / transmittance of viable cells would be estimated .