Integrated dystrophin analysis using immunocytochemical, biochemical and genetic techniques.

Basic and applied histochemistry Pub Date : 1990-01-01
L V Nicholson, M A Johnson, K E Davies
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Abstract

Immunocytochemistry is a powerful tool for the elucidation of clinical problems, particularly when used in conjunction with complementary techniques such as immunoblotting and DNA analysis. We would like to present an experimental example which illustrates the way these three different disciplines were brought together to investigate the defective gene product in X-linked muscular dystrophy.

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利用免疫细胞化学、生化和遗传技术综合分析肌营养不良蛋白。
免疫细胞化学是阐明临床问题的有力工具,特别是当与免疫印迹和DNA分析等补充技术结合使用时。我们想提出一个实验例子,说明这三个不同的学科是如何结合在一起研究x连锁肌营养不良症的缺陷基因产物的。
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Histochemical studies on irradiated and obstructive human submandibular glands. Protein distribution and lectin-binding in the degenerative glands. Cytochemical evaluation of C-heterochromatic-DNA in metaphase chromosomes. Integrated dystrophin analysis using immunocytochemical, biochemical and genetic techniques. Substance P- and calcitonin gene-related peptide-like immunoreactive pericellular baskets in human trigeminal ganglion. Phenylfluorone, a new staining agent with higher selectivity for tin.
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