Development of 3-Photon Functional Imaging in Deep Layers of Macaque Cortex

K. Takasaki, Gaku Hatanaka, Soumya Chatterjee, P. Balaram, Taekjun Kim, Celeste Dylla, Farzaneh Abolmaali, R. Reid, Anitha Pasupathy, W. Bair, Jack Waters
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Abstract

3-photon excitation laser-scanning microscopy enables fluorescence imaging deep in tissue beyond the range of 2-photon microscopy. Here, we report results of functional calcium imaging of neurons with 3-photon microscopy in non-human primates. We compare 2-photon and 3-photon excitation and characterize the performance of 3-photon microscopy at depths beyond the 2-photon limit.
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猕猴皮层深层三光子功能成像的研究进展
3光子激发激光扫描显微镜使荧光成像深入组织超出2光子显微镜的范围。在这里,我们报告了非人类灵长类动物神经元功能钙成像的3光子显微镜的结果。我们比较了2光子和3光子激发,并在超过2光子极限的深度下表征了3光子显微镜的性能。
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