Pub Date : 1900-01-01DOI: 10.1364/boda.2023.dtu3a.4
Roger Chiu Coutiño, David Gutiérrez-Hernández, Miguel S. Soriano-Garcia, R. Chiu
By using a convolutional neural network, we achieve recover images through skin phantoms. Our results demonstrate that using reinforced training is possible to reach thickness up to 3000 um. These results open the possibility for biomedical applications.
{"title":"Image dectection through skin phantom using convolutional neural network","authors":"Roger Chiu Coutiño, David Gutiérrez-Hernández, Miguel S. Soriano-Garcia, R. Chiu","doi":"10.1364/boda.2023.dtu3a.4","DOIUrl":"https://doi.org/10.1364/boda.2023.dtu3a.4","url":null,"abstract":"By using a convolutional neural network, we achieve recover images through skin phantoms. Our results demonstrate that using reinforced training is possible to reach thickness up to 3000 um. These results open the possibility for biomedical applications.","PeriodicalId":111173,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115386755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Maximiliano Mariné, Maximiliano V. Valdés, Vicente J Parot
Current single-shot multifocal imaging requires beam splitters or diffractive elements. We used a mirror cavity to capture an array of virtual images with offset focal depth on a camera. Focusing at multiple depths is demonstrated.
{"title":"Single-shot Multifocal Microscopy by Virtual Array Imaging","authors":"Maximiliano Mariné, Maximiliano V. Valdés, Vicente J Parot","doi":"10.1364/ntm.2023.nm2c.3","DOIUrl":"https://doi.org/10.1364/ntm.2023.nm2c.3","url":null,"abstract":"Current single-shot multifocal imaging requires beam splitters or diffractive elements. We used a mirror cavity to capture an array of virtual images with offset focal depth on a camera. Focusing at multiple depths is demonstrated.","PeriodicalId":111173,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)","volume":"48 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115670959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ondřej Vaculík, S. Bernatová, M. Sery, M. Šiler, O. Samek, K. Rebrošová, F. Ru̇žička, P. Zemánek
Analysis of bacteria in body fluids using Raman tweezers generates a mixture of signals from the bacteria and the medium. Advanced spectral unmixing algorithms (PCA, k-means, FIPPI, N-FINDR) can be used to separate each signal.
{"title":"Advanced Algorithms for Bacterial Analysis in Body Fluids Using Raman Tweezers","authors":"Ondřej Vaculík, S. Bernatová, M. Sery, M. Šiler, O. Samek, K. Rebrošová, F. Ru̇žička, P. Zemánek","doi":"10.1364/oma.2023.aw3d.2","DOIUrl":"https://doi.org/10.1364/oma.2023.aw3d.2","url":null,"abstract":"Analysis of bacteria in body fluids using Raman tweezers generates a mixture of signals from the bacteria and the medium. Advanced spectral unmixing algorithms (PCA, k-means, FIPPI, N-FINDR) can be used to separate each signal.","PeriodicalId":111173,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)","volume":"97 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121255895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.1364/ntm.2023.nth1c.6
Dante Maestre, Clara Conrad-Billroth, Felix Hitzelhammer, D. Bouchet, Jonathan Dong, T. Juffmann
In imaging, we retrieve information about a specimen by encoding it into a probe beam. We quantitatively analyze the Fisher information provided by phase and scattering microscopy techniques and discuss implications for microscope design.
{"title":"From information theory to novel imaging techniques","authors":"Dante Maestre, Clara Conrad-Billroth, Felix Hitzelhammer, D. Bouchet, Jonathan Dong, T. Juffmann","doi":"10.1364/ntm.2023.nth1c.6","DOIUrl":"https://doi.org/10.1364/ntm.2023.nth1c.6","url":null,"abstract":"In imaging, we retrieve information about a specimen by encoding it into a probe beam. We quantitatively analyze the Fisher information provided by phase and scattering microscopy techniques and discuss implications for microscope design.","PeriodicalId":111173,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)","volume":"178 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124743368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.1364/boda.2023.dth2a.6
Yaping Shi, Duo Zhang, Ruikang K Wang
we proposed a method to visualize light-evoked retinal response using swept source optical coherence tomography (SS-OCT). The framework here demonstrates the potential that a raster scanning SS-OCT may be feasible to measure the optoretinography response in the retina.
{"title":"In vivo optoretinography realized by raster-scan SS-OCT: A feasibility study","authors":"Yaping Shi, Duo Zhang, Ruikang K Wang","doi":"10.1364/boda.2023.dth2a.6","DOIUrl":"https://doi.org/10.1364/boda.2023.dth2a.6","url":null,"abstract":"we proposed a method to visualize light-evoked retinal response using swept source optical coherence tomography (SS-OCT). The framework here demonstrates the potential that a raster scanning SS-OCT may be feasible to measure the optoretinography response in the retina.","PeriodicalId":111173,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)","volume":"2021 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114911456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.1364/boda.2023.jw2b.2
Ye-chan Cho, Jungho Moon, Sungsam Kang, W. Choi
We introduce a method to record the transmission matrix of second-harmonic signals, and computationally correct sample-induced aberration. We demonstrated proposed concept by imaging a target under an aberration layer and visualizing muscle tissue in zebrafish.
{"title":"Second harmonic generation transmission matrix microscopy for computational aberration correction","authors":"Ye-chan Cho, Jungho Moon, Sungsam Kang, W. Choi","doi":"10.1364/boda.2023.jw2b.2","DOIUrl":"https://doi.org/10.1364/boda.2023.jw2b.2","url":null,"abstract":"We introduce a method to record the transmission matrix of second-harmonic signals, and computationally correct sample-induced aberration. We demonstrated proposed concept by imaging a target under an aberration layer and visualizing muscle tissue in zebrafish.","PeriodicalId":111173,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122415620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.1364/brain.2023.bm3b.2
J. Scherrer, G. F. Lynch, Jie J. Zhang, M. Fee
We present a fluorescence microscope design that enables imaging of thousands of cortical neurons in freely-moving mice. The design eliminates traditional illumination optics, allowing for head-mounted microscopes with both a lower weight (1.4 gram) and a larger field-of-view (~4 mm diameter) than previously possible.
{"title":"Next-generation head-mounted microscopes for large-scale neural recordings","authors":"J. Scherrer, G. F. Lynch, Jie J. Zhang, M. Fee","doi":"10.1364/brain.2023.bm3b.2","DOIUrl":"https://doi.org/10.1364/brain.2023.bm3b.2","url":null,"abstract":"We present a fluorescence microscope design that enables imaging of thousands of cortical neurons in freely-moving mice. The design eliminates traditional illumination optics, allowing for head-mounted microscopes with both a lower weight (1.4 gram) and a larger field-of-view (~4 mm diameter) than previously possible.","PeriodicalId":111173,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122118986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.1364/brain.2023.btu1b.4
Xiaodan Wang, A. Bice, A. Bauer
We created a novel method for mapping the interactions between parvalbumin inhibitory interneurons (PV-INs) and excitatory neurons over the cortex in mice. Local and distant influences of PV-INs are region-specific and can span hemispheres.
{"title":"Mapping Local and Global Interactions between Parvalbumin Inhibitory Neurons and Excitatory Neurons over the Cortex in Awake Mice","authors":"Xiaodan Wang, A. Bice, A. Bauer","doi":"10.1364/brain.2023.btu1b.4","DOIUrl":"https://doi.org/10.1364/brain.2023.btu1b.4","url":null,"abstract":"We created a novel method for mapping the interactions between parvalbumin inhibitory interneurons (PV-INs) and excitatory neurons over the cortex in mice. Local and distant influences of PV-INs are region-specific and can span hemispheres.","PeriodicalId":111173,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129069887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.1364/brain.2023.bw1b.5
Alexander J. Gray, Nathan Blanke, Rhiannon E Robinson, D. Rosene, I. Bigio
Structural imaging of myelin is of utmost importance for characterization of pathological changes to the brain in neurological disorders. Birefringence microscopy is used for rapid visualization of structural changes to myelin at sub-micron (<300nm) resolution.
{"title":"Birefringence Microscope Design and Sample Preparation Exigencies for High-resolution Imaging of Myelin Degradation","authors":"Alexander J. Gray, Nathan Blanke, Rhiannon E Robinson, D. Rosene, I. Bigio","doi":"10.1364/brain.2023.bw1b.5","DOIUrl":"https://doi.org/10.1364/brain.2023.bw1b.5","url":null,"abstract":"Structural imaging of myelin is of utmost importance for characterization of pathological changes to the brain in neurological disorders. Birefringence microscopy is used for rapid visualization of structural changes to myelin at sub-micron (<300nm) resolution.","PeriodicalId":111173,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)","volume":"44 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124004313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.1364/boda.2023.dm3a.5
Lara Pinar, A. Hielscher, S. H. Kim
This work addresses spatial resolution of parameterized temporal data types in time domain diffuse optical tomography (TD-DOT) based on the l0 norm. Various types of time filters, available from possible combinations of Mellin and Laplace transforms, were investigated in terms of their respective spatial resolution.
{"title":"Investigation of spatial resolution of parameterized temporal data in l0-norm based time-domain diffuse optical tomography (TD-DOT)","authors":"Lara Pinar, A. Hielscher, S. H. Kim","doi":"10.1364/boda.2023.dm3a.5","DOIUrl":"https://doi.org/10.1364/boda.2023.dm3a.5","url":null,"abstract":"This work addresses spatial resolution of parameterized temporal data types in time domain diffuse optical tomography (TD-DOT) based on the l0 norm. Various types of time filters, available from possible combinations of Mellin and Laplace transforms, were investigated in terms of their respective spatial resolution.","PeriodicalId":111173,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)","volume":"116 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124239810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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