On-site, parallel detection of bio-analytes in water by an integrated capillary flow based opto-microfluidic device

2015 IEEE International Symposium on Medical Measurements and Applications (MeMeA) Proceedings Pub Date : 2015-05-07 DOI:10.1109/MeMeA.2015.7145180

Nuno M. M. Pires, T. Dong

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Abstract

An integrated capillary-flow driven opto-microfluidic device for on-site, parallel detection of bio-analytes has been demonstrated in this work. An array of capillary-induced flow microchannels and chambers were arranged on a microfluidic chip made of poly(methyl methacrylate). This poly(methyl methacrylate) chip was further integrated with an array of eight ring-shaped organic photodiodes for measuring chemiluminescence of pathogen immunoassays. Highly sensitive detection of chemiluminescence was ensured by the use of gold nanoparticles, which were attached to the chambers of the microfluidic chip. The developed device demonstrated a detection limit in the range of ~104 cells/mL for bacteria detection in water. Furthermore, the device revealed feasibility to perform parallel detection of two types of bacteria and two types of viruses quantitatively. The presented concept may offer a promising route to realize truly in-the-field devices for medical and/or environmental measurements.

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现场，利用集成的毛细管流光学微流控装置平行检测水中生物分析物

一种集成的毛细管流驱动的光微流控装置，用于现场平行检测生物分析物。在聚甲基丙烯酸甲酯微流控芯片上设置了一系列毛细管诱导流动的微通道和微腔。该聚甲基丙烯酸甲酯芯片进一步集成了8个环形有机光电二极管阵列，用于测量病原体免疫分析的化学发光。利用金纳米颗粒附着在微流控芯片的腔室上，保证了化学发光的高灵敏度检测。该装置对水中细菌的检出限为~104个细胞/mL。此外，该装置还显示了对两种细菌和两种病毒进行定量平行检测的可行性。所提出的概念可能为实现用于医疗和/或环境测量的真正现场设备提供了一条有前途的途径。

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2015 IEEE International Symposium on Medical Measurements and Applications (MeMeA) Proceedings

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