[Stable erythrocyte reagents for detecting antigen-binding lymphocytes].

Abstract

Fixation of native chicken red cells with a solution containing 14.4 percent of acetate aldehyde and 0.85 percent of NaCl (pH 7.0-7.2) permitted preparation on the basis of these red cells of stable immunoreagents for the detection of antigen-fixing lymphocytes using any (optimal for the antigen used) procedure. Experiments have demonstrated a high efficacy of detecting staphylococcal, proteus, or Ps. aeruginosa etiology of pyoinflammatory and septic conditions in indirect rosette formation test with the developed immunoreagents. The levels of the detected antigen-fixing lymphocytes varied in the patients from 0.7 to 8.0 percent, the mean value being 2.1 +/- 0.1 percent.