Effect Of Irvingia Gabonensis Leaf Extracts On Non Pregnant Rat Uterus

The Internet Journal of Alternative Medicine Pub Date : 2009-12-31 DOI:10.5580/828

C. Nosiri, I. Hussaini, I. Abdu-Aguye

{"title":"Effect Of Irvingia Gabonensis Leaf Extracts On Non Pregnant Rat Uterus","authors":"C. Nosiri, I. Hussaini, I. Abdu-Aguye","doi":"10.5580/828","DOIUrl":null,"url":null,"abstract":"The effects of ethanol and water extracts of the leaves of Irvingia gabonensis on isolated non-pregnant rat uterus and Ileum have been investigated. The isolated uterine horns and Ileum each were mounted into organ baths containing different physiological solution connected to a recording microdynamometer to measure the contractions. The standard drugs Acetylcholine (Ach), Atropine, pirenzepine and verapamil and the extracts were injected into the organ bath to study their pharmacological effects on the uterine smooth muscles. Ethanol extract (25 400μg/ml) contracted the rat uterus while the water extract (0.025 – 20mg/ml ) had no effect. Both extracts had no observable contractile effect on rat ileum. The ethanol extract was less potent than acetylcholine in contracting the tissue. The response of the uterus to the ethanol extract was reduced by atropine (14nM ); Pirenzepine (0.1μM) and completely blocked by Verapamil ( 0.1μM ).This result indicates that the ethanol extract of Irvingia gabonensis may produce a ca++ dependent muscarinic receptor – mediated contraction (extracellular calcium dependent ) of the non pregnant rat uterus.","PeriodicalId":107168,"journal":{"name":"The Internet Journal of Alternative Medicine","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2009-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"4","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Internet Journal of Alternative Medicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5580/828","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 4

Abstract

The effects of ethanol and water extracts of the leaves of Irvingia gabonensis on isolated non-pregnant rat uterus and Ileum have been investigated. The isolated uterine horns and Ileum each were mounted into organ baths containing different physiological solution connected to a recording microdynamometer to measure the contractions. The standard drugs Acetylcholine (Ach), Atropine, pirenzepine and verapamil and the extracts were injected into the organ bath to study their pharmacological effects on the uterine smooth muscles. Ethanol extract (25 400μg/ml) contracted the rat uterus while the water extract (0.025 – 20mg/ml ) had no effect. Both extracts had no observable contractile effect on rat ileum. The ethanol extract was less potent than acetylcholine in contracting the tissue. The response of the uterus to the ethanol extract was reduced by atropine (14nM ); Pirenzepine (0.1μM) and completely blocked by Verapamil ( 0.1μM ).This result indicates that the ethanol extract of Irvingia gabonensis may produce a ca++ dependent muscarinic receptor – mediated contraction (extracellular calcium dependent ) of the non pregnant rat uterus.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

加蓬树叶提取物对未怀孕大鼠子宫的影响

研究了加蓬叶醇提物和水提物对离体非妊娠大鼠子宫和回肠的影响。将分离的子宫角和回肠分别装入含有不同生理溶液的器官浴中，并连接到记录微测力计以测量收缩。将标准药物乙酰胆碱(Ach)、阿托品、匹伦齐平、维拉帕米及其提取物分别注入器官浴，研究其对子宫平滑肌的药理作用。乙醇提取物(25 400μg/ml)对大鼠子宫有收缩作用，水提取物(0.025 ~ 20mg/ml)无收缩作用。两种提取物对大鼠回肠无明显收缩作用。乙醇提取物在收缩组织方面不如乙酰胆碱有效。阿托品(14nM)可降低子宫对乙醇提取物的反应;吡renzepine (0.1μM)和Verapamil (0.1μM)完全阻断。这一结果表明，加蓬藤的乙醇提取物可能产生一种ca++依赖的毒蕈碱受体介导的非妊娠大鼠子宫收缩(细胞外钙依赖)。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

The Internet Journal of Alternative Medicine

自引率

0.00%

发文量