APPLICATION OF DIFFERENTIAL MULLER-MATRIX TOMOGRAPHY OF HISTOLOGICAL SECTIONS OF STRUCTURED BIOLOGICAL HUMAN TISSUES TO DETERMINE THE DEGREE OF BLOOD LOSS

Abstract

In conducting a forensic medical examination of a corpse, in cases of acute blood loss, the decisive issue for investigators is to establish the amount of lost blood. However, this often remains a difficult task, since in the absence of a suitable quantitative system for measuring the volume of blood loss, forensic medical experts have to be guided by well-known morphological signs, which can be quite subjective. In conducting a forensic medical examination of a corpse, in cases of acute blood loss, the decisive issue for investigators is to establish the amount of lost blood. Therefore, it is important to find and develop new digital methods for determining the degree of blood loss. In order to solve this problem, the application of the method of multiparametric differential Mueller-matrix tomography of human biological tissues is proposed. Aim of the work. To develop a set of forensic criteria for the accurate determination of the volume of blood loss based on the data of differential Mueller-matrix tomography of histological sections of biological tissues with a fibrillar morphological structure. Materials and methods. Tissues with a fibrillar morphological structure (rectus abdominis muscle, skin) were collected from 60 cadavers of both sexes with varying degrees of blood loss from0 mm3to2500 mm3.The research was carried out by polarizing microscopy of histological sections of biological tissues in the standard location of the laser polarimeter. Results. Experimental measurements of distribution maps of linear birefringence of tissue sections with a fibrillar morphological structure with different degrees of blood loss were carried out. A linear range of changes in the magnitude of statistical moments of the 1st to 4th orders was established, which is associated with the degradation of linear birefringence of fibrillar networks of biological tissues. Conclusions. The effectiveness of applying the method of differential Mueller-matrix tomography of polycrystalline LD of the optically anisotropic component of structured biological tissues to changes in the level of blood loss of the deceased has been demonstrated. The sensitivity range ∆V=0÷1000 mm3 of this method is established.