Detección de ARN del SARS-CoV-2 por PCR en una serie de cadáveres remitidos para autopsia

Adriana C. D’Addario , Cristina A. Bustos , Roberto V. Cohen , Alejandro F. Rullán Corna , Patricia E. Gómez , Rubén H. Torrisi , Héctor Di Salvo , Jorge A. Herbstein , Federico Remes Lenicov , Santiago Maffia Bizzozero
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Abstract

Following the emergence of SARS-CoV-2 in the world, the COVID-19 pandemic broke out, affecting 185 countries. There have been numerous scientific publications presenting epidemiological, clinical, statistical, and microbiological data. The high mortality has implied the need for increasingly accurate data regarding the virus and its behaviour in the environment and, on the other hand, faced health teams with a serious problem in relation to the safe handling of corpses and the risks of transmission that this implies. The objective of this study was to detect the presence of SARS-CoV-2 RNA in corpses sent to the National Judicial Morgue that were not included in the definition of a suspected case submitted by the Argentine Ministry of Health. Nasopharyngeal and oropharyngeal swabs were taken from 101 cadavers chosen randomly and based on inclusion criteria for detection of viral RNA using the RT-PCR technique. Of the cadavers included in the study, 16.8%, not classified as suspected cases of COVID-19, were tested for the presence of viral RNA in the samples collected.

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继SARS-CoV-2在全球出现之后,新冠肺炎大流行爆发,影响185个国家。有许多科学出版物介绍了流行病学、临床、统计和微生物学数据。高死亡率意味着需要关于病毒及其在环境中的行为的越来越准确的数据,另一方面,卫生工作队面临着一个严重的问题,即如何安全处理尸体以及由此带来的传播风险。本研究的目的是检测送往国家司法停尸房的尸体中是否存在SARS-CoV-2 RNA,这些尸体未包括在阿根廷卫生部提交的疑似病例定义中。随机抽取101具尸体的鼻咽和口咽拭子,采用RT-PCR技术进行病毒RNA检测。在研究中包括的尸体中,16.8%未被归类为COVID-19疑似病例,他们在收集的样本中检测了病毒RNA的存在。
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来源期刊
Revista Espanola de Medicina Legal
Revista Espanola de Medicina Legal Medicine-Pathology and Forensic Medicine
CiteScore
1.90
自引率
0.00%
发文量
27
审稿时长
41 days
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