Laboratory Evaluation of Twenty Maize (Zea mays L.) Varieties for Resistance to the Larger Grain Borer, Ptosteptianus truncatus (Horn) {Coleoptera: BostIichidae}, in Ibadan, Oyo State, Nigeria

A. A. Osipitan, J. Odebiyi
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引用次数: 6

Abstract

Eighteen improved and two ‘local varieties ‘of maize (Zea mays L.) were evaluated for resistance to infestation by the larger grain borer, (LGB), Prostephanus truncatus (Horn);’ in the Entomology Research Laboratory of .the Department of Crop Protection and Environmental Biology, University of Ibadan, Oyo State, Nigeria at 28±1°C and 79-82% RH. -Sixty grammes of each variety were weighed into 250cm3 capacity Kilner jars and infested with 10 pairs of adult P. truncatus that were 1-5 days old. On the 40th day, all insects were collected, the maize grain dust sieved using a mesh of 0.25mm size and then ‘weighed. The’ grains were weighed and sorted into, damaged and undamaged categories and percentage damage computed, Other data recorded were the percentage grain weight loss, adult mortality and final population of adults. The study was conducted using completely randomized design and the test varieties were compared with standard using Dunnett’s t-test. The weighted average of scores generated from the five damage parameters was used to place the .varieties into one, of four rating for resistance to LGB. In addition, cluster analysis was done on the rated parameters using the centroid. hierarchical system. The results indicated that ICZBC2-B (KUI414) was highly resistant; TZBR-Ses-3C3, TZBR-Ses-IC3-Y, TZBR~Syn-YC2′ Oba Super-I and Oba Super-l l were resistant; ‘TZBR-Eld-3C2, Ama-TZBR-WC1′, TZBR-Ses-3C4-Y’ Ak- 9528-DMR-SR, Acr.91-Suwan-ISRC were moderately resistant, while others were susceptible. The mechanisms of resistance identified were antixenosis ‘and antibiosis, and the basis of resistance, were grain hardness and starch content. The resistant varieties could be used in combination with other control options in LGB-endemic areas.
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20种玉米(Zea mays L.)的实验室评价尼日利亚奥约州伊巴丹省抗大谷物螟虫Ptosteptianus truncatus(角){鞘翅目:BostIichidae}的品种
在尼日利亚奥约州伊巴丹大学作物保护与环境生物学系昆虫学研究实验室,对18个改良玉米和2个“地方品种”玉米(Zea mays L.)在28±1°C和79-82%相对湿度条件下对较大谷物螟虫(LGB) Prostephanus truncatus (Horn)的抗性进行了评估。每个品种60克称重装入容量为250cm3的Kilner罐中,并用10对出生1-5天的成虫进行侵染。第40天采集所有昆虫,用0.25mm筛网筛取玉米粒粉并称重。对籽粒进行称重和分类,分为受损和未受损两类,并计算受损百分比,记录籽粒重量损失率、成虫死亡率和最终成虫数量。本研究采用完全随机设计,试验品种与标准品种采用Dunnett t检验进行比较。从5个危害参数中产生的分数加权平均值被用来将品种划分为抗LGB的4个等级中的一个。此外,利用质心对额定参数进行聚类分析。分级系统。结果表明,ICZBC2-B (KUI414)具有高抗性;TZBR- ses - 3c3、TZBR- ses - ic3 - y、TZBR~Syn-YC2′′对Oba super - 1和Oba Super-l - 1具有抗性;‘tzbr - old - 3c2’、Ama-TZBR-WC1’、tzbr - sds - 3c4 - y’、Ak- 9528-DMR-SR、Acr.91-Suwan-ISRC为中抗性,其余为易感。抗性机制主要为抗异源性和抗菌素性,抗性的基础是籽粒硬度和淀粉含量。在lgb流行地区,抗药品种可与其他控制方案结合使用。
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