Analysis of genetic divergence in Durum wheat (Triticum Durum Desf.) and selection of elite parents for hybridization

Arega Gashaw, H. Mohammed, Harjit Singh
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Abstract

A field experiment was conducted at Geregera and Kone testing sites of Sirinka Agricultural Research Center, northeastern Ethiopia. The objective of the experiment was to estimate the genetic divergence among durum wheat germplasm of diverse origin and clustering them into homogenous groups for further hybridization program. Genetic divergence analysis was computed based on multivariate analysis using Mahalanobis's D 2 statistics. Based on D2 values, 64 durum wheat genotypes were grouped into ten clusters. The highest inter-cluster distance was exhibited between cluster-II and cluster-III (D 2 =57.15). Analysis within the indigenous durum wheat germplasm indicated that there was no correspondence between geographic and genetic distances. That is, germplasm collected from the same geographic area were placed into different cluster groups indicating their differences. Thus, to get more genetic variability, further collection mission should be targeted in major durum wheat growing regions of Ethiopia. On the other hand, indigenous and exotic germplasm were grouped into different clusters except in cluster-VI and cluster-X. Cluster-VI consisted of seven indigenous and one exotic germplasm. Cluster-X on the contrary, consisted of seven exotic and one indigenous germplasm, implying the presence of parallelism between genetic and geographic distances. Thus, there is an opportunity to improve grain yield through hybridization of genotypes from genetically divergent clusters and subsequent selection from the segregating generations. Crossing of parents involving cluster-IX (indigenous) with cluster-III (exotic) would complement each other and could result in high genetic variability and superior segregates having good combinations of characters from both parents. Keywords/phrases: Clustering, Genetic divergence, Genetic variability Ethiop. J. Biol. Sci ., 7(2): 111-121, 2008
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硬粒小麦(Triticum Durum Desf.)遗传分化分析及杂交优良亲本选择
在埃塞俄比亚东北部Sirinka农业研究中心的Geregera和Kone试验点进行了田间试验。本试验的目的是评估不同来源硬粒小麦种质间的遗传分化,并将其聚类为同质类群,以便进行进一步的杂交计划。遗传差异分析采用马氏d2统计量进行多元分析。根据D2值,将64个硬粒小麦基因型分为10个聚类。聚类ii和聚类iii之间的簇间距离最大(d2 =57.15)。对本地硬粒小麦种质资源的分析表明,地理距离和遗传距离之间没有对应关系。即将同一地理区域的种质放在不同的聚类组中,以显示其差异。因此,为了获得更多的遗传变异,进一步的收集任务应针对埃塞俄比亚硬粒小麦主产区。另一方面,本地和外来种质除聚类vi和聚类x外,均被划分在不同的聚类中。集群vi包括7个本地种质和1个外来种质。相反,簇x由7个外来种质和1个本地种质组成,这意味着遗传距离和地理距离之间存在平行性。因此,有机会通过从遗传不同的群体中进行基因型杂交和随后从分离的世代中进行选择来提高粮食产量。亲本ix(本土)与亲本iii(外来)杂交,可以形成高遗传变异性和优良分离,亲本双方性状组合良好。关键词:聚类;遗传分化;遗传变异;生物。科学通报,7(2):111-121,2008
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