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Durability of Ethiopian bamboo culms and alternative damage control measures against biodeteriorating agents 埃塞俄比亚竹秆的耐久性和针对生物变质剂的替代损害控制措施
Pub Date : 2015-12-30 DOI: 10.4314/EJBS.V14I2
G. Desalegn
High susceptibility of the bamboo stems (culms) to biodeteriorating agents are among the major challenges in processing, value addition, marketing and rational utilization of the bamboo resource in Ethiopia. A study was conducted to  investigate culms’ natural durability, treatability of bamboo culms with and effectiveness of damage control measures in controlling the biodeteriorating agents’ damage. Samples of Arundinaria alpina culms were collected from Hagere Selam, Injibara, Tikur Inchini and Masha sites. Oxytenanthera abyssinica samples were obtained from Asossa, Dedessa and Pawe. Tanalith preservative, borax-boric acid solution, used motor oil, kerosene and common table salt were used to treat the bamboo stakes. Graveyard studies were conducted for five years at Pawe, Bako, Adami Tulu and Addis Ababa research stations. The non-ground contact test was conducted in Addis Ababa station under shade without direct contact with soil, moisture, rain and sunlight. The results indicated that bamboo culms were non-durable, having high treatability potential with the control measures used in this study. Significant difference (P<0.01) was found on damage caused by subterranean termites on the bamboo species stakes obtained from different localities, control measures and field (graveyard) stations. Mean damage on controls and treated stakes with control measures caused by subterranean termites for all stations varied from 24 to 80%, while damage caused by fungi varied from 11 to 66%. This indicated that termites caused the highest damage at all stations both on the controls and treated stakes. The non-ground contact stakes were intact against aboveground dwelling termites, beetles and fungi. Tanalith and used motor oil treatments were found to be the most effective alternative control measures in resisting biodegrading agents attack and prolonged bamboo culms service life to more than five times compared with controls. Keywords: Arundinaria alpina , Control measures, Culms, Graveyard stations, Oxytenanthera abyssinica , Stakes
竹茎(秆)对生物变质剂的高度敏感性是埃塞俄比亚竹子资源加工、增值、销售和合理利用的主要挑战之一。研究了竹秆的自然耐久度、可处理性以及防治措施对生物腐坏剂危害的效果。在Hagere Selam、Injibara、Tikur Inchini和Masha等地采集了冬青秆样品。在阿索萨、德德萨和帕韦地区采集了深草样品。竹桩的处理方法主要有Tanalith防腐剂、硼酸溶液、废机油、煤油和普通食盐。墓地研究在Pawe、Bako、Adami Tulu和亚的斯亚贝巴研究站进行了五年。非地面接触试验在亚的斯亚贝巴站阴凉处进行,不直接接触土壤、水分、雨水和阳光。结果表明,竹秆不耐久,在本研究的防治措施下具有很高的处理潜力。地下白蚁对不同地区、不同防治措施、不同田间(墓地)站位的竹种桩的危害有显著差异(P<0.01)。地下白蚁对所有站点的对照和采取控制措施的处理桩造成的平均损害从24%到80%不等,而真菌造成的损害从11%到66%不等。这表明白蚁在所有站点对对照和处理过的木桩造成的损害最大。非地面接触木桩对地上居住的白蚁、甲虫和真菌完好无损。结果表明,与对照相比,Tanalith和废机油处理是抗生物降解剂侵袭最有效的替代防治措施,可将竹竿的使用寿命延长5倍以上。关键词:高山雪莲,防治措施,秆,墓地站,深海雪莲,木桩
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引用次数: 7
Overview of the flora of Ethiopia and Eritrea: The long road to a completion 埃塞俄比亚和厄立特里亚植物区系概述:漫长的完成之路
Pub Date : 2014-10-17 DOI: 10.4314/EJBS.V13I1S
S. Demissew
Prior to the initiation of the Ethiopian Flora Project (EFP), there were a number of attempts over the years to write the flora for a particular region/s or areas of Ethiopia and Eritrea. The modern Ethiopian Flora Project was initiated in 1980 as a bilateral agreement between the Ethiopian and Swedish governments through the then Ethiopian Science and Technology Commission (ESTC) (now the Ministry of Science and Technology) and the Swedish Agency for Research Cooperation with Developing Countries (SAREC) (now Sida-SAREC). The project was carried out with shared responsibilities between Addis Ababa University and Uppsala University, Sweden. The objectives of the project were: 1) to write up a Flora of Ethiopia (FE), which later became the Flora of Ethiopia and Eritrea (FEE) with the separation of Eritrea from Ethiopia in 1991; 2) build-up of the National Herbarium and a related library; and 3) promotion of scientific activities in other fields of botany. By the end of the project in 2009, the writing of the FEE was completed with the publication of 8 volumes in 10 books including information on the description, ecology and distribution of 6,027 vascular plants taxa. Of these taxa, 10% are endemic to Ethiopia and Eritrea (Ensermu Kelbessa and Sebsebe Demissew, 2014 in this volume). Due to this project, the number of plant specimen in the Herbarium grew from 14,000 in 1980 to 80,000 at present. The project has also trained 11 Ethiopian nationals at PhD level, who later become the nucleus for the advancement of Botanical Sciences in Ethiopia. The early conception of the complementary value of a Botanical Garden to the National Herbarium for research and education by these young academicians led to the initiation of its establishment. They had worked tirelessly over the years. This effort finally led to the establishment of the Gulelle Botanic Gardens on the outskirts of Addis Ababa in 2005 in collaboration with the Addis Ababa City Administration. Key words/phrases: Botany, Ethiopian Flora Project, Gulelle Botanical Garden, National Herbarium
在埃塞俄比亚植物区系计划(EFP)启动之前,多年来有许多人试图为埃塞俄比亚和厄立特里亚的一个特定地区或地区编写植物区系。现代埃塞俄比亚植物项目于1980年启动,是埃塞俄比亚和瑞典政府通过当时的埃塞俄比亚科学技术委员会(ESTC)(现在的科技部)和瑞典与发展中国家研究合作机构(SAREC)(现在的Sida-SAREC)达成的双边协议。该项目由亚的斯亚贝巴大学和瑞典乌普萨拉大学共同负责。该项目的目标是:1)编写一份埃塞俄比亚植物志(FE),该植物志后来在1991年厄立特里亚从埃塞俄比亚分离后成为埃塞俄比亚和厄立特里亚植物志(FEE);2)建立国家植物标本室和相关图书馆;3)促进其他植物学领域的科学活动。到2009年项目结束时,FEE的编写工作已经完成,出版了8卷10本,包括6027个维管植物分类群的描述、生态和分布信息。在这些分类群中,10%是埃塞俄比亚和厄立特里亚特有的(Ensermu Kelbessa和Sebsebe Demissew, 2014年在本卷中)。由于这个项目,植物标本室的植物标本数量从1980年的14000件增加到现在的80000件。该项目还培训了11名具有博士学位的埃塞俄比亚人,他们后来成为埃塞俄比亚植物学发展的核心。这些年轻的学者们早期就认识到植物园对国家植物标本室的研究和教育具有互补的价值,从而促成了植物园的建立。这些年来他们不知疲倦地工作着。这一努力最终促成了2005年与亚的斯亚贝巴市政府合作,在亚的斯亚贝巴郊区建立了古丽植物园。关键词:植物学;埃塞俄比亚植物计划;古雷勒植物园
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引用次数: 1
Gillnet selectivity of Lake Tana piscivorous fish: Labeobarbus megastoma 塔纳湖鱼食性鱼的刺网选择性:巨骨钩骨鱼
Pub Date : 2014-10-11 DOI: 10.4314/EJBS.V13I1
B. Abdissa
Gillnet selectivity parameters and its impacts on the population of the Lake Tana Labeobarbus megastoma , were estimated from fishery-independent catches in multi-panel gillnets with stretched mesh sizes ranging from 60 to 140 mm. Selectivity on L. megastoma was assessed using the PASGEAR software. Five different functional models; normal location, normal scale, gamma, log-normal and bi-modal were used to fit the selectivity curves to the catch data. It was shown that the bi-modal function was the best fit to the data. For the bi-modal selectivity curves, the optimum lengths (100% retention probability) for the 60, 80, 100, 120 and 140 mm mesh sizes were 24.18 cm, 32.24 cm, 40.3 cm, 48.36 cm and 56.42 cm, respectively. Considering the size at first maturity (L 50 %), 100 mm mesh size was found to be the most adequate mesh for exploiting the minimum allowable landing size (35 cm). Key words/phrases: Bi-modal, Labeobarbus , Lake Tana, Selectivity
利用60 ~ 140 mm拉伸网目尺寸的多板刺网,估算了刺网选择性参数及其对塔纳湖拉比奥巴布巨细胞瘤种群的影响。应用PASGEAR软件对乳状瘤的选择性进行评估。五种不同的功能模型;采用正态位置、正态尺度、伽马、对数正态和双峰等方法拟合选择性曲线。结果表明,双峰函数与实测数据最拟合。对于双峰选择性曲线,60、80、100、120和140 mm孔径的最佳长度(100%保留率)分别为24.18 cm、32.24 cm、40.3 cm、48.36 cm和56.42 cm。考虑到初成熟时的尺寸(l50%),发现100 mm的网孔尺寸是利用最小允许着陆尺寸(35 cm)的最合适的网孔。关键词:双模态,Labeobarbus, Lake Tana,选择性
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引用次数: 0
Artificial propagation of Clarias gariepinus (Burchell, 1822) in aquaria 水族箱中Clarias gariepinus (Burchell, 1822)的人工繁殖
Pub Date : 2014-07-01 DOI: 10.4314/EJBS.V14I1
Adane Tsegaye Tegegne, A. Bekele, M. Balakrishnan
Induced spawning of the African catfish (Clarias gariepinus) was successfully carried out using natural hormone (homoplastic hormone-pituitary extract from Clarias gariepinus). The study which was carried out at Bahir Dar Fisheries and Other Aquatic Life Research Centre lasted 78 days (June to October). Three gravid females and three mature males of C. gariepinus (weight range of 305 to 1035 g) were used for the study. In all, three trials were carried out in glass aquaria with 50 litre volume. The spawning fecundity of the three C. gariepinus injected with catfish pituitary extract varied from 43,456 to 75,460 with mean fecundity value 54,572. The mean percentage fertilization, hatching rate and survival rate of the eggs were 81.5 ± 2.36, 87.13 ± 0.13 and 87.04 ± 5.98, respectively. This study has shown that C. gariepinus can be successfully bred using pituitary of C. gariepinus with simple low cost technique using glass aquaria.Key words/phrases: Aquaria, Catfish, Fecundity, Induced spawning, Survival rate.
采用天然激素(同质激素-垂体提取物)对非洲鲶鱼(Clarias gariepinus)进行了诱导产卵试验。这项在Bahir Dar渔业和其他水生生物研究中心进行的研究持续了78天(6月至10月)。研究对象为3只妊娠雌鱼和3只成年雄鱼,体重范围为305 ~ 1035 g。总共有三次试验是在容积为50升的玻璃水族箱中进行的。注射鲶鱼垂体提取物后,3只加里波波维奇鲤的产卵繁殖力在43,456 ~ 75,460之间变化,平均繁殖力为54,572。卵受精率、孵化率和成活率分别为81.5±2.36、87.13±0.13和87.04±5.98。本研究表明,在玻璃水族箱中采用简单、低成本的技术,可以成功地培育出加里滨鲟。关键词:水族,鲶鱼,繁殖力,诱导产卵,存活率
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引用次数: 5
Long-term changes in phyto- and zooplankton communities of Lake Hawassa, Ethiopia 埃塞俄比亚哈瓦萨湖植物和浮游动物群落的长期变化
Pub Date : 2014-03-04 DOI: 10.4314/EJBS.V13I2
T. Fetahi, S. Mengistou
We studied phyto- and zooplankton communities of Lake Hawassa (or Awassa) between November 2003 and August 2004, and compared findings with historical data since the 1980s to assess potential inter-decadal changes. The lake is located in the Ethiopian Rift Valley in the vicinity of the growing city Hawassa which receives adverse effluents from textile and ceramics industry and municipal sewage apparently with little treatment. In 2003/04, phytoplankton abundance comprised 54% Chlorophyta, 26% cyanoprokaryotes, 18% diatoms and 2% others, a proportion similar to previous records. However, the mean phytoplankton biomass in terms of chlorophyll a (19 μg L -1 ) was lower than reported from previous studies but similar to a report in 2010. In contrary, areal rate of gross photosynthesis had increased in the last two decades with higher values recorded in 2003-04 (0.35 to 2.21 g O 2 m -2 h -1 ). Even though zooplankton community composition remained the same, the abundance and dominance of taxa had changed in the last decades. In 2003/04, the mean abundance of cyclopoid-copepods was 58 000 ± 9200 (SE) Indl m -3 whereas cladocera abundance (2 600 ± 640 SE Indl m -3 ) was very low in the lake. Rotifers outnumbered other zooplankton with a maximum value of 264 000 Indl m -3 ,which was about five times greater than previous reports. Adult cyclopoid to nauplii ratio of 0.27 in 2003-04 study was indicative that cannibalism had diminished, probably due to increased rotifers as prey, which contradicted a previous hypothesis. Despite its closed nature, the plankton of Lake Hawassachanged erratically during a decade, probably due to intense biological interactions in the system.
我们研究了2003年11月至2004年8月期间哈瓦萨湖(或阿瓦萨湖)的植物和浮游动物群落,并将研究结果与20世纪80年代以来的历史数据进行了比较,以评估潜在的年代际变化。该湖位于埃塞俄比亚大裂谷,靠近不断发展的城市哈瓦萨,该城市接收来自纺织和陶瓷工业的有害废水以及显然未经处理的城市污水。在2003/04年度,浮游植物丰度包括54%的绿藻,26%的蓝藻原核生物,18%的硅藻和2%的其他,这一比例与以前的记录相似。然而,以叶绿素a为单位的浮游植物平均生物量(19 μg L -1)低于以往的研究报告,但与2010年的报告相似。相反,总光合作用的面积速率在过去20年中有所增加,2003-04年录得较高的数值(0.35 ~ 2.21 g O 2 m -2 h -1)。尽管浮游动物群落组成保持不变,但在过去几十年中,分类群的丰度和优势度发生了变化。2003/04年,环足类-桡足类平均丰度为58 000±9200 (SE) inl m -3,枝足类平均丰度为2 600±640 (SE) inl m -3,丰度极低。轮虫的数量超过其他浮游动物,最高值为264 000 Indl m -3,约为以前报告的5倍。2003-04年的研究表明,成虫的同类相食行为减少了,这可能是由于作为猎物的轮虫增加了,这与之前的假设相矛盾。尽管它是封闭的,但哈瓦萨湖的浮游生物在十年内发生了不规律的变化,这可能是由于该系统中强烈的生物相互作用。
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引用次数: 2
FOOD AND FEEDING HABITS OF THE STRAIGHTFIN BARB BARBUS PALUDINOSUS (PETERS, 1852) (PISCES: CYPRINIDAE) IN LAKE ZIWAY, ETHIOPIA 衣索比亚齐威湖直刺鱼barbus paludinosus (peters, 1852)(双鱼座:鲤科)的食物和摄食习性
Pub Date : 2013-09-25 DOI: 10.4314/EJBS.V12I2
E. Dadebo, A. Mohammed, S. Sorsa
The feeding habits of the straightfin barb Barbus paludinosus  was studied in Lake Ziway, Ethiopia, from 504 gut samples collected from April to May (dry months) and July to August (wet months) of the year 2011. Gut contents were analyzed using the frequency of occurrence and volumetric analysis methods. Insects and detritus were the dominant food items and they occurred in 86.3% and 93.5%, and constituted 47.3% and 38.0%, of the total volume of food items, respectively. Macrophytes, phytoplankton, zooplankton and ostracods occurred in 36.1%, 40.3%, 32.7% and 15.5% of fish guts and volumetrically they constituted 4.8%, 4.7%, 3.9% and 1.3% of the food items, respectively. Insects, detritus and zooplankton were important food categories in the dry season while detritus, insects and phytoplankton dominated in the wet season. Insects were the dominant food items in all size classes followed by detritus. The proportion of macrophytes increased with size of fish while the importance of detritus and zooplankton declined with fish size. Based on these results, it was concluded that B. paludinosus in Lake Ziway has an omnivorous habit.  Keywords: B. paludinosus , Diet composition, Fish feeding habit, Lake Ziway, Ontogenetic diet shifts.
采用2011年4 ~ 5月(旱季)和7 ~ 8月(雨季)采集的504份肠道样本,对埃塞俄比亚齐韦湖直刺Barbus paludinosus的摄食习性进行了研究。采用发生频率和体积分析法分析肠道内容物。昆虫和碎屑是主要食物,分别占总食物量的86.3%和93.5%,占总食物量的47.3%和38.0%。大型植物、浮游植物、浮游动物和介形虫分别占鱼类内脏的36.1%、40.3%、32.7%和15.5%,占食物种类的4.8%、4.7%、3.9%和1.3%。干季以昆虫、碎屑物和浮游动物为主,湿季以碎屑物、昆虫和浮游植物为主。昆虫是所有大小类别的主要食物,其次是碎屑。大型植物的比例随着鱼的大小而增加,而碎屑和浮游动物的重要性随着鱼的大小而降低。基于以上结果,兹威湖的白藻具有杂食性。关键词:白鲟;饲料组成;鱼类摄食习性;
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引用次数: 2
A review of coffee wilt disease, Gibberella xylarioides (Fusarium xylarioides) in Africa with special reference to Ethiopia 非洲咖啡枯萎病木耳赤霉病(Fusarium xylarioides)的研究进展,特别是埃塞俄比亚
Pub Date : 2012-09-21 DOI: 10.4314/EJBS.V11I1
T. Alemu
Coffee is vital to the economy of East and Central Africa,providing a major source of foreign exchange earnings and as a cash crop,supporting the livelihood of millions of people who are involved in cultivation, processing, marketing, and export. Coffee is attacked by variousdisease-causing organisms such as fungi, bacteria, viruses, nematodes, insects and weeds. One of the limiting factors for coffee production in Central and East African countries is tracheomycosis/vascular wilt disease caused by Fusarium xylarioides Steyaert imperfect stage (Gibberella xylarioides Heim and Saccas perfect stage). Coffee production and development is now threatened by coffee wilt disease (CWD). The major difference between tracheomycosis and many other coffee diseases is that it kills all affected trees at all stages of growth. Coffee wilt disease was first observed in 1927 in a plantation of Coffea excelsa, in the Central African Republic. Since then, CWD has re-emerged on C. canephora/excelsa in portions of the Democratic Republic of Congo in the mid-1980s, it affected up to 90% of plantations in 1993 in Uganda. The fungus lives in the soil, on infected debris, in alternative hosts or as resistant propagules of species, and enters the coffee tree through wounds at the base of the tree or on the roots. The outbreak of the pathogen has been reported throughout the major coffee-growing woredas in the south and south western parts of Ethiopia. The disease infestation incidence varied between 14.9 and 34.0%. The estimated annual coffee yield losses caused by CWD are about 7.4%, 1.6% and 2.6% in Uganda, Ethiopia and Tanzania, respectively. CWD is distributed, and caused coffee yield losses in major coffee-growing areas of western, southern and eastern parts of Ethiopia. The mean disease incidence ranged from 45% at Gera to 69% at Bebeka, with certain variations between coffee fields at each locality. The pathogen survives in the soil. It is difficult to control the pathogen by fungicides. However, the pathogen may be controlled by antagonistic biological control agents. In vitro evaluation of Trichoderma species has revealed up to 71% reduction of the mycelial growth of coffee wilt pathogen (F. xylarioides).
咖啡对东非和中非的经济至关重要,是外汇收入的主要来源,也是一种经济作物,支持数百万从事种植、加工、销售和出口的人的生计。咖啡会受到各种致病生物的侵害,比如真菌、细菌、病毒、线虫、昆虫和杂草。制约中非和东非国家咖啡生产的因素之一是由木镰刀菌Steyaert不完全期(木镰刀菌Heim和Saccas完美期)引起的气管霉菌病/血管性枯萎病。咖啡枯萎病(CWD)对咖啡的生产和发展构成威胁。气管霉菌病和许多其他咖啡疾病的主要区别是,它会在生长的各个阶段杀死所有受影响的树木。咖啡枯萎病于1927年首次在中非共和国的一个咖啡种植园被发现。从那时起,CWD在20世纪80年代中期在刚果民主共和国的部分地区再次出现在canephora/excelsa上,1993年在乌干达影响了高达90%的种植园。这种真菌生活在土壤中、被感染的碎片上、替代宿主中或作为物种的抗性繁殖体,并通过树底部或根部的伤口进入咖啡树。据报告,在埃塞俄比亚南部和西南部的主要咖啡种植区都爆发了这种病原体。侵染率为14.9% ~ 34.0%。据估计,在乌干达、埃塞俄比亚和坦桑尼亚,CWD造成的年咖啡产量损失分别为7.4%、1.6%和2.6%。CWD在埃塞俄比亚西部、南部和东部的主要咖啡种植区分布,并造成咖啡产量损失。平均发病率从Gera的45%到Bebeka的69%不等,每个地方的咖啡田之间存在一定差异。病原体在土壤中存活。用杀菌剂控制病原菌是困难的。然而,这种病原菌可能被拮抗生物防治剂所控制。木霉对咖啡枯萎病病原菌(F. xylarioides)菌丝生长的体外评价显示,木霉对咖啡枯萎病病原菌菌丝生长的抑制作用可达71%。
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引用次数: 9
Microbiological study of Wakalim, a traditional Ethiopian fermented sausage 埃塞俄比亚传统发酵香肠Wakalim的微生物学研究
Pub Date : 2009-08-31 DOI: 10.4314/EJBS.V6I2.45457
K. Bacha, T. Mehari, M. Ashenafi
ABSTRACT: Wakalim is a traditional Ethiopian fermented beef sausage prepared and consumed commonly in Harar, Eastern Ethiopia. It has good keeping quality and the traditional processing technique is applicable at the household level. In this study, detailed information pertaining to the preparation processes and the raw materials used for its preparation was documented. Moreover, the safety quality of the ready-to-eat product was evaluated through microbiological analysis of the product as it was availed to the local consumers. The raw materials required for preparation of wakalim consist of lean meat (70%), fat (5%), salt (2%), garlic (1%), onion (17%) and other spices (5%). Wakalim samples were found to be dominated by aerobic mesophilic bacteria (AMB), mainly aerobic spore formers (ASF), followed by lactic acid bacteria (LAB) and staphylococci, with mean counts (log cfu/g) of 6.02, 4.70, and 4.59, respectively. Species of LAB isolated from wakalim samples mainly consisted of Pediococcus pentosaceus 1 (29%), Lactobacillus plantarum 1 (19%), Ped. pentosaceus 2 (17%), Lb. brevis 1 (16%), Lactococcus lactis ssp. lactis (6%), Lb. pentosus (4%), Lb. brevis 3 (3%), and other Lactobacillus and Pediococcus species (6%). The average pH and titratable acidity of wakalim samples were 5.35 and 6.4%, respectively.
摘要:Wakalim是一种埃塞俄比亚传统的发酵牛肉香肠,常见于埃塞俄比亚东部的哈拉尔。其保藏性好,传统的加工工艺适用于家庭生产。在本研究中,详细的信息有关的制备过程和用于其制备的原料被记录。此外,即食食品在提供给本地消费者时,经微生物分析,评估其安全品质。制作wakalim所需的原料包括瘦肉(70%)、脂肪(5%)、盐(2%)、大蒜(1%)、洋葱(17%)和其他香料(5%)。Wakalim样品中以好氧中温细菌(AMB)为主,以好氧孢子形成菌(ASF)为主,其次为乳酸菌(LAB)和葡萄球菌,平均计数(log cfu/g)分别为6.02、4.70和4.59。从瓦卡林样品中分离到的乳酸菌种类主要为戊糖Pediococcus penttosaceus 1(29%)、植物乳杆菌Lactobacillus plantarum 1(19%)、Ped。pentosaceus 2 (17%), Lb. brevis 1 (16%), Lactococcus lactis;乳酸菌(6%),戊酸杆菌(4%),短杆菌3(3%),以及其他乳杆菌和Pediococcus(6%)。瓦卡林样品的平均pH值为5.35,可滴定酸度为6.4%。
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引用次数: 9
Phenotypic characteristics of common bean (Phaseolus vulgaris)-nodulating rhizobia from some parts of southern Ethiopia 埃塞俄比亚南部部分地区普通豆(Phaseolus vulgaris)根瘤菌的表型特征
Pub Date : 2009-08-31 DOI: 10.4314/EJBS.V6I2.45454
A. Workalemahu, F. Assefa
ABSTRACT: Eighteen strains of common bean rhizobia from Konso (GI), Arbaminch (GII), Sodo (GIII) and Ziway-Awassa-Dilla (GIV) areas in southern Ethiopia were isolated. The strains were characterized by sixty-four phenotypic traits. These traits were tested for cluster analysis using unweighted pair group method with average (UPGMA) with NTSYS version 2.1. pH tolerance/sensitivity, antibiotic tolerance/sensitivity together with the numerical analysis results clearly showed diversity among the strains. The cluster analysis grouped the strains into two clusters that included 78% of the strains at 85% similarity level, except four strains which were not clustered. These strains were less related to the clustered strains and to each other. The clustered stains were with the presumed similar characters of Rhizobium leguminosarun/Rhizoium etli group except AUPR10. Two isolates, AUPR9 (unclustered) and AUPR10 (cluster I), exhibited rough colony appearance on Peptone Yeast Extract Agar medium (PY) that resemble the characteristics of Rhizobium gallicum . Strain AUPR8 (from unclustered strains) displayed creamy colony appearance on PY, tolerance to extreme pH, salt, and temperature and inability to utilize dulcitol and utilization of glycine that resembled with characters of Rhizobium tropici . This result, therefore, indicates the possibility of obtaining inoculant strains with tolerance to environmental conditions of common bean-producing areas of Ethiopia.
摘要:从埃塞俄比亚南部Konso (GI)、Arbaminch (GII)、Sodo (GIII)和Ziway-Awassa-Dilla (GIV)地区分离到18株普通豆根瘤菌。菌株具有64个表型性状。采用NTSYS 2.1版本的非加权平均对群法(UPGMA)对这些性状进行聚类分析。pH耐受性/敏感性、抗生素耐受性/敏感性以及数值分析结果明显显示出菌株间的多样性。聚类分析将菌株分为2个聚类,其中78%的菌株在85%的相似水平上聚类,其余4个菌株未聚类。这些菌株与聚集株之间的相关性较小,彼此之间的相关性也较小。聚群染色除AUPR10外,均与豆科根瘤菌/根瘤菌群具有相似的性状。两个分离株AUPR9(非聚类)和AUPR10(聚类I)在蛋白胨酵母提取物琼脂培养基(PY)上表现出粗糙的菌落外观,与高芝根瘤菌的特征相似。菌株AUPR8(来自未聚集的菌株)在PY上表现出奶油菌落外观,对极端pH、盐和温度的耐受性,不能利用dulcitol和甘氨酸,与热带根瘤菌的特征相似。因此,这一结果表明获得对埃塞俄比亚普通豆产区环境条件具有耐受性的接种菌株的可能性。
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引用次数: 3
Seroprevalence of caprine brucellosis in Chifra Woreda, Afar Regional State, Ethiopia 埃塞俄比亚阿法尔州奇夫拉沃勒达地区山羊布鲁氏菌病的血清患病率
Pub Date : 2009-08-31 DOI: 10.4314/EJBS.V6I2.45459
Abraham Ali, A. Mesfin, E. Yimer, A. Deressa, T. Girmay
ABSTRACT: A cross sectional study was carried out from December 2005 to June 2006 to investigate seroprevalence of caprine brucellosis in Afar Regional State of Chifra Woreda, Ethiopia. Of 294 serum samples screened by RBPT, 62 (21%) were positive. When the RBPT positive (n=62) sera were retested with Complement Fixation Test (CFT), only 54 (18.4%) became positive. There was significant difference in seroprevalence among the three age groups (P difference (P>0.05) in seroprevalence between the two sexes, high prevalence (19.6%) was observed in male goats. The present study also demonstrates a statistically significant association between abortion (P seropositivity. The results found in this study appear to be related with management system practiced in the area.
摘要:本研究于2005年12月至2006年6月在埃塞俄比亚奇夫拉·沃勒达阿法尔地区调查山羊布鲁氏菌病的血清流行情况。在294份经RBPT筛选的血清样本中,62份(21%)呈阳性。当RBPT阳性(n=62)血清再次进行补体固定试验(CFT)时,只有54例(18.4%)呈阳性。3个年龄组间血清阳性率差异有统计学意义(P>0.05),其中公山羊阳性率较高(19.6%)。本研究还表明流产与P血清阳性之间有统计学意义的关联。本研究发现的结果似乎与该地区实行的管理制度有关。
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引用次数: 2
期刊
Ethiopian Journal of Biological Sciences
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