Investigating the Anti-diabetic Effect of Leucaena leucocephala Linn. (Ipil-Ipil) Seed Extract on Albino Mice

Abstract

An experiment utilizing the Randomized Complete Block Design (RCBD) was done to prove the anti-diabetic effect of Leucaena leucocephala Linn. (Ipil-ipil) seed extract on albino mice as test animals. The study also aimed to detect the phytochemicals present in the extract, ascertain its physical properties, and compare its effect among three concentration levels and the positive control, a commercially prepared anti-hyperglycemic drug.Analysis of the extract‟s chemical properties showed the presence of alkaloid, flavonoid, saponin, tannin, and phenolic compounds; anthraquinone and steroid were not detected. The extract‟s color was orange, with a pleasant odor, a pH of 5.44, and was found to be miscible in both water and acetone, but was immiscible in chloroform. Among the three extract concentration levels used, 50% level was found to be most effective in reducing blood glucose levels (BGL) in the experimental animals, while the pure extract (100% concentration) was the least effective. However, reductions in BGL were not significantly different among the concentration levels and that of the commercial drug. Thus, it is clear that Leucaena leucocephala Linn has anti-diabetic potentials which are comparable to the commercial anti-hyperglycemic drug Metformin.It is recommended that other parts of the plant be also tested for its anti-diabetic potentials taking into consideration also the detection of other secondary metabolites which may be present in the plant parts, and evaluating its other potential applications.