Studies on exopolysaccharide production from Aureobasidium pullulans spi 10 and its application as a green inhibitor for corrosion mitigation

Kajal Mahadev Gaikwad, Geetanjali Eknath Pansare, Monika Prashant Madane, Y. Mulay
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Abstract

The corrosion of metals potentially reduces the shelf life of the infrastructure assets. Hence, it is necessary to reduce the corrosion to save considerable amount in structure. The organic corrosion inhibitors show the environmental toxicity which urged search for eco-friendly green corrosion inhibitors. Exopolysaccharide from Aureobasidium pullulans was studied with regard to its potential application as green inhibitor of corrosion. Isolation of A. pullulans was carried out from spinach. Biochemical and molecular characterizations of A. pullulans spi 10 were carried out. Pullulan production was maximum on fifth day of incubation. Pullulan precipitation was carried out by using isopropyl alcohol. The study of the corrosive stability of steel samples was conducted by the gravimetric method. The rate of corrosion, the degree of protection, and coefficient of protection were studied. With pullulan coating, the rate of corrosion was 0.348 × 10 −3 g/cm 2 .hour, which is approximately five times less than that of corrosion rate of steel plate without any protection (control). The degree of protection shown by biofilm ( A. pullulans) is 24%, whereas the degree of protection governed by pullulan is 77%. The results reveal that biofilm of A. pullulans spi 10 and pullulan are both reliable and environment friendly green inhibitors that mitigate corrosion.
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普鲁兰毛霉spi 10产胞外多糖的研究及其作为缓蚀剂的应用
金属的腐蚀可能会缩短基础设施资产的保质期。因此,有必要减少腐蚀,以节省相当多的结构费用。有机缓蚀剂表现出环境毒性,迫切需要寻找环保型绿色缓蚀剂。研究了普鲁兰毛霉胞外多糖作为绿色缓蚀剂的应用前景。从菠菜中分离出普鲁兰单胞菌。对普鲁兰sp10进行了生化和分子表征。普鲁兰产量在孵化第5天达到最大值。用异丙醇沉淀普鲁兰。用重量法对钢试样的腐蚀稳定性进行了研究。研究了腐蚀速率、防护程度和防护系数。普鲁兰涂层的腐蚀速率为0.348 × 10−3 g/ cm2 .h,比未加防护(对照)的钢板的腐蚀速率约低5倍。普鲁兰菌生物膜的保护程度为24%,普鲁兰菌的保护程度为77%。结果表明,普鲁兰菌的生物膜spi 10和普鲁兰菌是一种可靠的、环境友好的绿色缓蚀剂。
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