Journal of Applied Biology & Biotechnology最新文献

{"title":"In vitro antioxidant and acetylcholinesterase activities of catechin-loaded green fabricated zinc oxide nanoparticles","authors":"Nandhini Baskaran, Anitha Subash","doi":"10.7324/jabb.2023.131095","DOIUrl":"https://doi.org/10.7324/jabb.2023.131095","url":null,"abstract":"","PeriodicalId":423079,"journal":{"name":"Journal of Applied Biology & Biotechnology","volume":"183 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115133427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation on the antifungal activity of Aspergillus giganteus in different culture conditions","authors":"S. Karthiga, R. Ramya, K. Ramya, S. Jothinayaki, D. Kavitha","doi":"10.7324/jabb.2022.100514","DOIUrl":"https://doi.org/10.7324/jabb.2022.100514","url":null,"abstract":"Systemic fungal diseases are therapeutic crises which additionally have high mortality rates in case of deferred treatment. Nowadays, antimicrobial potential of several natural compounds have been demonstrated in a wider aspects. This study investigates the antagonist efficacy of Antifungal Proteins (AFP) from Aspergillus giganteus to treat fungal infections. The antagonistic efficacy of Aspergillus giganteus is determined by performing dual culturing and was further proved by hyphal interactions. The culture conditions for maximum antagonistic effect at different pH, temperature, carbon and, nitrogen sources were optimized. The antagonistic potential of AFP against the human pathogen Aspergillus flavus was highly influenced by various culture conditions. The level of inhibition by Afp was 84%, when dextrose was used as the carbon source along with (NH4)H2PO4 as the nitrogen source. The antagonistic was enhanced at pH 7 as 85% in while 80% inhibition was attained at the temperature 28°C. The inhibitory potential of AFP from Aspergillus giganteus can be used as a lead compound for development of antifungal drugs.","PeriodicalId":423079,"journal":{"name":"Journal of Applied Biology & Biotechnology","volume":"40 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114555446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Industrial biotechnology: An Indian perspective","authors":"Kumud Tiwari, G. Singh, Gajender Singh, Sonika Sharma, S. Singh","doi":"10.7324/jabb.2022.100503","DOIUrl":"https://doi.org/10.7324/jabb.2022.100503","url":null,"abstract":"Advancement in the field of biotechnology has opened a vast global market. The Indian biotechnology arena is promising for advance and pioneering growth with its immense growth potential which could play a significant role toward India’s contribution to global industrial biotechnology. Today, India is one of the fastest-growing trillion-dollar economies in the world and the fifth largest overall, with a GDP (gross domestic product) of $2.94 trillion. Biotechnology (BT) and information technology (IT) are the key drivers contributing to this growth which constitutes approximately 5% of the country’s total annual GDP. Indian biotechnology growth is fueled by bio-pharmaceutical, bio-industrial, bio-services, bio-agricultural, and bioinformatics, and among them, the bio-industrial area is one of the most promising and advanced. New approaches which Indian industrial biotechnology is exploring, include harnessing microorganisms for the production of value-added bioactive ingredients such as industrial enzymes, organic acids, bulk chemicals, and single-cell proteins, which have played a predominant role in the overall development of biotechnology after bio-pharmaceuticals. The above factors made India one of the world’s top 12 destinations for biotechnology. In this article, we review the status of the biotechnological industry and its future perspective in context to the Indian market and its role in global economy.","PeriodicalId":423079,"journal":{"name":"Journal of Applied Biology & Biotechnology","volume":"69 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127605875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In-vitro investigation of cholesterol removal, ß-galactosidase synthesis, antioxidant, and antidiabetic potential of probiotic organisms","authors":"Jahanvee Chanpura, Shilpa Gupte","doi":"10.7324/jabb.2022.100521","DOIUrl":"https://doi.org/10.7324/jabb.2022.100521","url":null,"abstract":"The present study is aimed to determine some important health beneficial properties of probiotic isolates such as cholesterol removal, β -galactosidase production, antioxidant, and anti-diabetic activity. Nine probiotic isolates were screened for Bile Salt Hydrolase (BSH) activity and potential BSH producers were selected for cholesterol removal study. Isolate B11 has shown significant cholesterol removal ability (88 ± 0.2%) after 24 h and this property was also analyzed with variable concentrations, time profile, with live, and dead cells as well as from egg yolk with simulation treatment. As probiotics improve lactose intolerance, all isolates were assessed for their lactose utilizing and β -galactosidase production ability where maximum enzyme activity was observed in case of isolate D25 (226 ± 0.30 Miller units). All the isolates were also assessed for their antioxidant potential using four different methods such as reducing power assay, 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid, 2,2-diphenyl-1-picrylhydrazyl assay, and superoxide dismutase activity. Out of nine isolates, isolate D25 and B11 have shown significant antioxidant property. Further, these isolates were also subjected to anti-diabetic study, where isolate D25 (89 ± 0.01%) and B11 (98 ± 0.30%) have shown high α -glucosidase inhibition which indicate their effective anti-diabetic activity. Hence, overall probiotic isolate D25 and B11 have shown significant health beneficial properties and they were further identified as Lactiplantibacillus plantarum and Pediococcus pentosaceus , respectively.","PeriodicalId":423079,"journal":{"name":"Journal of Applied Biology & Biotechnology","volume":"81 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127623408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive reviews on phenolic compounds from Phaeophyceae as potential therapeutic agent","authors":"V. Maheswari, P. S. Babu","doi":"10.7324/jabb.2022.100502","DOIUrl":"https://doi.org/10.7324/jabb.2022.100502","url":null,"abstract":"Seaweeds are an excellent source of natural bioactive compounds. The exploration of novel natural compounds from marine resources has gained interest lately which possesses greater pharmaceutical and nutritional values. Seaweed phenolic compounds, particularly phlorotannin, have been discovered to have a variety of biological implications. Phlorotannin is a polyphenol that is found majorly in brown seaweed and is made up of polymeric units of phloroglucinol. The structural configuration and degree of polymerization were shown to influence biological activity. Several in vitro studies demonstrated that the phlorotannin derivatives had substantial bioactivity and were moderately appraised in vivo . Antioxidant, anticancer, anti-inflammatory, anti-allergic, anti-diabetic, and anti-microbial effects have been discovered in phlorotannin compounds. Recently, they have been evaluated for exhibiting anti-viral capacity against various harmful viruses. The findings suggested that phlorotannin could be an effective anti-viral molecule that requires intense research. This review focuses on the advanced techniques and research based on the experiments on phlorotannin for their extraction and purification. The phlorotannin as a potential drug molecule has been described from extraction to application. With the advent of technology, it is now possible to isolate the target molecule efficiently in less time. To make phlorotannin a novel nutraceutical and pharmaceutical molecule with wide industrial uses, preclinical and clinical research is required to assess its efficacy, toxicity, bioavailability, and drug delivery mechanism.","PeriodicalId":423079,"journal":{"name":"Journal of Applied Biology & Biotechnology","volume":" 47","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133019847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In silico identification of target fetal protein(s) in the development of polycystic ovarian syndrome","authors":"Pawan Kumar, Deepak Deepak, V. Saini, A. Annu, N. Yadav, Ajit Kumar","doi":"10.7324/jabb.2022.100522","DOIUrl":"https://doi.org/10.7324/jabb.2022.100522","url":null,"abstract":"Polycystic ovarian syndrome (PCOS) is a multifactorial reproductive disorder mainly affecting ovulating women. Animal studies to date have identified hyperandrogenicity as one of the major causes of PCOS, while estrogen treatment temporarily decreases symptoms. Researchers believe that a high androgen level in a pregnant woman during pregnancy results PCOS-like symptoms in the newborn female baby, which are expressed later during reproductive age. The present work is an in silico analysis of the effect of hyperandrogenicity during fetal ovarian development. An alteration in the level of steroid hormone (androgen, estrogen, progesterone, and testosterone) reportedly affects gonadotropin-releasing hormone (GnRH) secretion and later on the development of ovarian follicles. Nuclear receptors such as DAX-1 (dosage-sensitive sex reversal, adrenal hypoplasia congenital critical region on the X chromosome, gene 1) and steroidogenic factor-1 (SF-1) also affect the level of various sex hormones and ovary formation. Hence, the study was carried out to evaluate the effect of these steroid hormones on the promoter region of KISS1 gene and DAX1 gene apart from the effect of these steroid hormones on the binding of kisspeptin (involved in GnRH secretion) and SF1 protein (involved in DAX1 expression modulation). The study involved molecular modeling of promoter regions of KISS1 and DAX1 gene; kisspeptin and SF1 proteins, followed by molecular dockings studies of these promoter regions and proteins against steroid hormone (androgen, estrogen, progesterone, and testosterone), taken as ligand. The study reflected that both the androgen and progesterone show binding over the TATA box of the KISS1 gene, which can be inferred to possibly regulate its expression and affect GnRH secretion to imbalance hypothalamus-pituitary-gonadal axis. This alteration may further cause an abnormal luteinizing hormone: Follicle-stimulating hormone ratio that may result in abnormal steroidogenesis. The molecular docking studies of SF1 protein against DAX1 promoter region were observed to be better than binding when SF1 protein was complexed with studied steroid hormones. The observations lead to the inference that binding of steroid hormones with SF1 protein lowers the expression of DAX1 gene, as the former is essentially required for DAX1 gene expression, which may result in abnormal ovary development in a female fetus as well as abnormal sex steroids level. Thus, it may be concluded that over-secretion of sex steroids is likely to affect female fetus development and hypothalamus-pituitary-gonads axis to trigger PCOS-like symptoms.","PeriodicalId":423079,"journal":{"name":"Journal of Applied Biology & Biotechnology","volume":"63 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117163944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of butterfly pea flower extract in processing some Vietnamese traditional foods","authors":"N. Thuy, T. Ben, P. Ngoc, N. V. Tai","doi":"10.7324/jabb.2022.100518","DOIUrl":"https://doi.org/10.7324/jabb.2022.100518","url":null,"abstract":"Butterfly pea flower (BPF) extract was applied to the processing of traditional Vietnamese food products to bring attractiveness and safety. Different cooking techniques as steaming, boiling, frying, baking, and sterilization were used and controlled. All products were analyzed for total anthocyanin content remaining after processing conditions. The selected percentage of extract and treatment time (minutes) in different processing methods were obtained: Bloating fern-shaped cake/thin cakes (bánh bèo) (10% extract – steaming time 7 min); Rice ball sweet soup – chè ỉ) (15% extract – boiling time 6 min), fried Meat stuffed rice ball – viên thịt) (10% extract (v/w) – frying time 4 min), and bread (15% extract – baking temperature 160°C). Half-life values of anthocyanin degradation at different temperatures (through cooking methods) were observed. A case study was performed with BPF drink, this product had high quality when adding 5% BPF extract (v/v), sterilized at 125°C for 3 min with extended storage time (more than 4 months). The degradation of anthocyanin during storage was modeled according to the first-order kinetics. The half-life values were calculated as 38 and 46 weeks, respectively, at ambient (28 ± 2°C) and low temperature (6 ± 2°C) storage.","PeriodicalId":423079,"journal":{"name":"Journal of Applied Biology & Biotechnology","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126359622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanotechnology for the bioremediation of heavy metals and metalloids","authors":"Urja Sharma, J. Sharma","doi":"10.7324/jabb.2022.100504","DOIUrl":"https://doi.org/10.7324/jabb.2022.100504","url":null,"abstract":"Contamination of soil and water by heavy metals and metalloids is one of the major issues that are being raised and addressed globally as it has adverse effects on the environment as well as on human health. Since each technique has its own pros and cons, integration of a few methods helps in getting effective and efficient results. Application of nanotechnology has led to the overcoming of various drawbacks of conventional methods of remediation. Nanobioremediation is an extended branch of nanotechnology that deals with the removal of pollutants from the site of contamination by utilizing biogenic nanoparticles or materials synthesized from biological sources that are of nano size. This technique has an edge over other methods because of size of the material; smaller the size, higher would be the surface area to volume ratio and higher the ratio, more surface would be available for the reaction to occur. In recent years, the green synthesis of nanoparticles has gained enormous attention because of the economic and ecological aspects. This review highlights the implications and health risks of heavy metals and metalloids along with the application of nanotechnology in the bioremediation of these contaminants.","PeriodicalId":423079,"journal":{"name":"Journal of Applied Biology & Biotechnology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130476520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emerging microplastic contamination in ecosystem: An urge for environmental sustainability","authors":"Akanksha Saini, J. Sharma","doi":"10.7324/jabb.2022.100508","DOIUrl":"https://doi.org/10.7324/jabb.2022.100508","url":null,"abstract":"Plastic use has increased steadily in recent years due to the urbanization and industrialization at global scale. Due to the population expansion, more plastic products are being utilized in today’s scenario. Most of the plastic waste generated is due to its single usage that finds its pathway in oceans, seas, rivers, ponds, and landfills. Plastic waste on degradation produces microsized plastic (diameter <5 mm) termed as microplastics (MPs). MP contamination in environment is facilitated through various sources including cosmetic products, drug carriers, glitters, and disintegration of larger plastic products such as water bottles and fishing net. Due to their ubiquitous use in the environment, they possess serious threat to terrestrial and aquatic environments and human health. Many countries have already established regulations such as ban of single-use plastics and Microbeads-Free Waters Act to control its pollution and impacts on organisms. This review explores thoroughly the interactions of MPs with other pollutants, toxicological effects of the MP additives, occurrences of MPs, and impacts on the soil stability, structure, organisms, marine species, plants, and human health. This review also covers the strategies and regulations that are implemented to mitigate the MPs pollution.","PeriodicalId":423079,"journal":{"name":"Journal of Applied Biology &amp; Biotechnology","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132520295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of different cooking conditions on resistant starch and estimated glycemic index of macaroni","authors":"N. Thuy, N. V. Tai","doi":"10.7324/jabb.2022.100519","DOIUrl":"https://doi.org/10.7324/jabb.2022.100519","url":null,"abstract":"Resistant starch is a type of carbohydrate that is slowly digested in the small intestine and fermented in the large intestine. Studies have been conducted to investigate the effects of different cooking methods (boiling, steaming, microwave, stir-frying, and deep frying), cooling and freezing on the quality of macaroni prepared with wheat flour and other resistant combinations starch sources. In this study, the in vitro digestibility of macaroni was determined and the glycemic index was estimated. Research results showed that cooking methods (boiling, steaming, microwave, stir-frying, and deep frying) reduced the resistant starch content of macaroni from 3.37 to 66.66%; however, cooling and freezing significantly increased the resistant starch content of macaroni from 6.88 to 24.19% and 9.85 to 37.28%, respectively. Macaroni prepared with the addition of flour/starch containing high levels of resistant starch exhibited a significantly lower estimated glycemic index (44.53–47.10) than the control sample using100% wheat flour (49.31).","PeriodicalId":423079,"journal":{"name":"Journal of Applied Biology &amp; Biotechnology","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128962705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}