Determination of cytoplasmic 5'-nucleotidase which preferentially hydrolyses 6-hydroxypurine nucleotides in pig, rat and human tissues by immunotitration.

R Itoh, K Yamada
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Abstract

1. Activity of a 5'-nucleotidase which preferentially hydrolyses IMP and GMP was determined by immunotitration in various mammalian tissues. 2. Activity per g of rat tissue was high in testis and spleen and low in skeletal muscle. 3. In human cells, the activity was high in fibroblasts and low in erythrocytes.

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免疫滴定法测定猪、大鼠和人组织中优先水解6-羟嘌呤核苷酸的细胞质5′-核苷酸酶。
1. 用免疫滴定法测定了多种哺乳动物组织中优先水解IMP和GMP的5′-核苷酸酶的活性。2. 每克大鼠睾丸和脾脏组织的活性高,骨骼肌组织的活性低。3.在人类细胞中,成纤维细胞活性高,红细胞活性低。
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