Determination of cytoplasmic 5'-nucleotidase which preferentially hydrolyses 6-hydroxypurine nucleotides in pig, rat and human tissues by immunotitration.
{"title":"Determination of cytoplasmic 5'-nucleotidase which preferentially hydrolyses 6-hydroxypurine nucleotides in pig, rat and human tissues by immunotitration.","authors":"R Itoh, K Yamada","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>1. Activity of a 5'-nucleotidase which preferentially hydrolyses IMP and GMP was determined by immunotitration in various mammalian tissues. 2. Activity per g of rat tissue was high in testis and spleen and low in skeletal muscle. 3. In human cells, the activity was high in fibroblasts and low in erythrocytes.</p>","PeriodicalId":22539,"journal":{"name":"The International journal of biochemistry","volume":"23 4","pages":"461-5"},"PeriodicalIF":0.0000,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"The International journal of biochemistry","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
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Abstract
1. Activity of a 5'-nucleotidase which preferentially hydrolyses IMP and GMP was determined by immunotitration in various mammalian tissues. 2. Activity per g of rat tissue was high in testis and spleen and low in skeletal muscle. 3. In human cells, the activity was high in fibroblasts and low in erythrocytes.