Protein-Based Detection Methods for Genetically Modified Crops

K. Malik, H. Sadia, M. Basit
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引用次数: 4

Abstract

The generation of genetically modified (GM) crops is rapidly expanding each and every year around the world. The well-being and quality assessment of these harvests are vital issues with respect to buyers’ interests. This drove the administrative specialists to execute an arrangement of extremely strict strategies for the endorsement to develop and use GMOs and to produce an interest in scientific techniques equipped for identifying GM crops. The GM crops have been added to the effective fuse of various attributes by presenting transgenes, for example, Bacillus thuringiensis (Bt) insecticidal qualities, in various crop species. GM crops give critical financial, natural, well-being and social advantages to both small and large agriculturists. The detection strategies incorporate either DNA-based or protein-based measures. Different immunoassays or catalyst connected immunosorbent tests are delicate and more affordable; however, they need experienced technicians. A very simple method, that is, immunochromatographic (ICS) test, is set up in the world, which is modest, compact and simple to utilize. The ICS is a semiquantitative method for indicative screening and semi-measurement of new remote proteins presented through hereditary change of plants. The strip is the easiest method for the assessment of several Bt crop plants for insecticidal quality.
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基于蛋白质的转基因作物检测方法
转基因作物的生产每年都在世界范围内迅速扩大。这些收获的福祉和质量评估是对买家利益至关重要的问题。这促使行政专家执行了一项极其严格的战略安排,以批准开发和使用转基因生物,并对用于识别转基因作物的科学技术产生兴趣。转基因作物通过在各种作物中表现出转基因,例如苏云金芽孢杆菌(Bt)杀虫特性,从而有效地融合了各种特性。转基因作物给小农户和大农户带来了重要的经济、自然、福祉和社会优势。检测策略包括基于dna或基于蛋白质的测量。不同的免疫分析或催化剂连接的免疫吸附试验是精细和更实惠的;然而,他们需要经验丰富的技术人员。国际上建立了一种非常简单的方法,即免疫层析(ICS)检测,该方法体积小、结构紧凑、操作简单。ICS是一种通过植物遗传变化对新的远程蛋白进行指示性筛选和半定量测定的半定量方法。条带法是评价几种Bt作物杀虫质量最简便的方法。
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Cellular Interaction of Human Eukaryotic Elongation Factor 1A Isoforms Protein-Based Detection Methods for Genetically Modified Crops Introductory Chapter: Protein-Protein Interactions and Assays A Novel Protein-Protein Interaction Assay Based on the Functional Complementation of Mutant Firefly Luciferases: Split Structure Versus Divided Reaction
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