Mie scattering characterization by ultra-spectral illumination using micro-sized tunable liquid crystal optical filters

F. Vasefi, R. Barbour, C. Stoltzfus, Z. Barber, D. Farkas
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引用次数: 1

Abstract

We developed a confocal microscope and ultra-spectral light source containing Liquid Crystal Arrayed Microcavities (LCAM) as a new technology for controlled ultra-narrow optical filtering (FWHM ∼0.1 nm). LCAMs use picoliter volume Fabry–Perot-type optical cavities filled with liquid crystal for wavelength tuning. With sub-nanometer spectral resolution, we measured spectral resonance from scattering particles to validate the measurements with simulation results. We measured chicken liver and breast muscle, pig skin and muscle and compared with results from literature. The main application is the estimation of nuclear size in biological tissues for noninvasive assessment equivalent to histopathology.
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利用微尺寸可调谐液晶滤光片的超光谱照明表征米氏散射
我们开发了一种共聚焦显微镜和含有液晶阵列微腔(LCAM)的超光谱光源,作为控制超窄光学滤波(FWHM ~ 0.1 nm)的新技术。lcam使用皮升体积的法布里-珀罗式光学腔填充液晶进行波长调谐。在亚纳米光谱分辨率下,我们测量了散射粒子的光谱共振,并通过仿真结果验证了测量结果。我们测量了鸡肝和胸肌、猪皮和肌肉,并与文献结果进行了比较。主要应用是估计核大小的生物组织的非侵入性评估相当于组织病理学。
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Fully packaged confocal endomicroscopic system using Lissajous fiber scanner for indocyanine green in-vivo imaging Mie scattering characterization by ultra-spectral illumination using micro-sized tunable liquid crystal optical filters Highly-sensitive quantitative thermal microscopy using gold nano-particles as heat sources
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