Biochemical changes of the Liver and Kidney of Albino Mice Mus musculus Exposed to Organphosphorus insecticide Imidacloprid (Commando 30%)

Abstract

This study planned to assess the toxicity, biochemical investigation associated with hepatotoxicity and nephrotoxicity of insecticide Imidacloprid (Commando 30%) in addition to serum cholinesterase (chE) activity in albino mice at orally administered sublethal dose of Commando 1/20 of LD50. Results revealed that The LD50 values obtained at 24, 48, 72 and 96 hours exposures and the 95% confidence limits for the imidacloprid (Commando 30%) obtained from the sigmoid curve were 495, 437, 344 and 210 mg/kg bw, respectively. the net body weight gains of the animals intoxicated with sublethal doses of Commando 30% was markedly significant decrease (P≤ 0.5). Significant reduction (P ˂ 0.05) in total protein, and significant increase in ALT, AST, ALP and LDH enzyme activity levels as well as significant decrease in kidney function (urea and creatinine) in all the experimental groups of mice. Serum Cholinestrase (ChE) activity was markedly significant decrease by Commando exposure mice at sublethal dose. The blood parameters of all tested groups, results showed that the median values of blood parameters decreased in animals treated with Commando 30% at a dose of 10.5 mg/kg body weight, indicative of anemic syndrome. In addition, there was a significant reduction in the number of erythrocytes and of hemoglobin concentration, with reduced hematocrit and increased MCV, characteristic of macrocytic anemia.