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Heliculture of garden snail (Helix aspersa) inhabiting Egypt using different food items 居住在埃及的花园蜗牛(螺旋aspersa)的螺旋栽培,使用不同的食物
Pub Date : 1900-01-01 DOI: 10.5455/egysebz.20190626085601
W. Salama, M. Mona, Mohamed A. El Disouky, Amal M. Mohamadeen, A. Fahmy
Helix aspersa is one of the dominant snail species grown in Egypt. total of 400 garden snails were used in this study, harvested from garden in new Cairo, Egypt. Collected samples were separated into 5 groups of 30 snails per treatment. The remaining groups were fed with different feeding diets as follows; carrot, flour mix (wheat flour 60%, corn flour 20%, CaCO3 20%), pumpkin and cucumber. Six snails were weighed every week throughout the experiment. After 6 weeks, 6 snails from each treatment were cleaned prior their preparation for analysis. Protein, ash, lipid, fiber, moisture, carbohydrates and amino acid composition were determined in all samples. The results showed that the groups of snails which fed on carrot, pumpkin showed significant increase in the total protein level. The ash contents in the groups of snails which fed on carrot, pumpkin or mix flour were increased when compared to the field snails. Total lipids in the group of snails that fed on pumpkin or mix flour significantly decreased when compared to the group of snails that fed on carrot or those collected from the field. Significant decrease in the total fibers were observed in the group of snails that fed on carrot, pumpkin or mix flour when compared to their level in the field snails. Total carbohydrates in the group of snails that fed on carrot, pumpkin or mix flour showed significant reduction after 5 weeks of feeding. Total amino acids showed their high values in groups that fed on carrot and pumpkin. It was calculated that carrot feeding improve the biochemical composition and provide rich nutritional values of investigated snail.
螺旋螺是生长在埃及的主要螺种之一。本研究共使用400只花园蜗牛,采自埃及新开罗的一个花园。将采集的钉螺分为5组,每组30只。其余各组分别饲喂如下饲粮;胡萝卜,面粉(小麦粉60%,玉米粉20%,碳酸钙20%),南瓜和黄瓜。在整个实验过程中,每周对6只蜗牛进行称重。6周后,各处理6只钉螺清洗后准备分析。测定了所有样品的蛋白质、灰分、脂肪、纤维、水分、碳水化合物和氨基酸组成。结果表明,以胡萝卜、南瓜为食的钉螺组总蛋白质水平显著提高。以胡萝卜、南瓜和混合粉为食的钉螺组灰分含量均高于田间钉螺。与吃胡萝卜或田间采集的蜗牛相比,吃南瓜或混合面粉的蜗牛的总脂质显著降低。与田间蜗牛相比,食用胡萝卜、南瓜或混合面粉的蜗牛的总纤维量显著减少。喂食胡萝卜、南瓜或混合面粉的蜗牛组,在喂食5周后,总碳水化合物显著减少。以胡萝卜和南瓜为食组的总氨基酸含量较高。结果表明,饲喂胡萝卜改善了蜗牛的生化组成,提供了丰富的营养价值。
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引用次数: 0
Phytochemical screening and in vitro antitumor effect of Datura stramonium seed and leave extracts 曼陀罗种子及叶提取物的植物化学筛选及体外抗肿瘤作用
Pub Date : 1900-01-01 DOI: 10.5455/egysebz.20220106090421
S. Naggar, A. Bossery, Abeer gyar, S. Nassar
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引用次数: 0
Spermatogenesis of the Small-Scaled Terapon, Terapon puta, from Lake Timsah, Ismailia Governorate, Egypt 来自埃及伊斯梅利亚省Timsah湖的小型Terapon puta的精子发生
Pub Date : 1900-01-01 DOI: 10.5455/egysebz.20191103120216
W. Rizkalla, H. Shabaka, Fawzia Rahman, M. Naggar
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引用次数: 0
Effect of wheat germ oil on phenylhydrazine-induced toxicity in male albino rats 小麦胚芽油对苯肼致雄性白化大鼠毒性的影响
Pub Date : 1900-01-01 DOI: 10.5455/egysebz.20230108094930
M. Hegazy
Wheat germ oil (WGO) is a natural source of antioxidant such as glucosylated hydroquinones, tocopherols and it is an excellent source of polyunsaturated fatty acids. The point of the present study is to see how efficient is the WGO against phenylhydrazine (PHZ) induced hemotoxicity in rats. Rats were divided into five groups (six rats each); control group (normal rats), WGO group (administrated by daily oral dose of WGO, 300 mg/kg b.w.), PHZ group (injected intraperitoneally with a daily dose of PHZ, 60 mg/kg b.w. for 3 consecutive days), treated group (injected with PHZ then after 3 days administered by WGO) and protective group (administrated daily by WGO for 11 days then injected with PHZ). The experimental trial was continued for a total period of 14 days. The results showed that the PHZ induced significant decreases in hematological parameters (RBCs, Hb, Hct, PLT), serum proteins (total protein, albumin and globulin) and antioxidant enzymes (CAT, GPX and SOD) activity and significant increases in white blood cells (WBCs), erythropoietin hormone, serum ferritin level, serum liver function parameters (ALT, AST, TBIL and ALP), serum kidney function parameters (creatinine, urea and uric acid) and malondialdehyde (MDA) content. Administration of WGO improved the hematological parameters, erythropoietin hormone, ferritin level, serum proteins, liver function enzymes, kidney function parameters, antioxidant enzyme activities and MDA content in rats injected with PHZ. In conclusion, the results of the present study revealed that the WGO has a therapeutic and protective roles in improving the hemolytic anemia and toxicity induced by PHZ.
小麦胚芽油(WGO)是一种天然的抗氧化剂,如糖基化对苯二酚,生育酚,它是一个极好的来源多不饱和脂肪酸。本研究的重点是观察WGO对苯肼(PHZ)诱导的大鼠血液毒性的有效性。大鼠分为五组(每组6只);对照组(正常大鼠)、WGO组(WGO每日口服剂量,300 mg/kg体重)、PHZ组(PHZ每日腹腔注射剂量,60 mg/kg体重,连续3天)、治疗组(注射PHZ,连续3天给药)和保护组(WGO每日给药,连续11天注射PHZ)。试验期共14 d。结果表明:PHZ诱导血液学指标(红细胞、Hb、Hct、PLT)、血清蛋白(总蛋白、白蛋白和球蛋白)和抗氧化酶(CAT、GPX和SOD)活性显著降低,白细胞(wbc)、促红细胞生成素激素、血清铁蛋白水平、血清肝功能指标(ALT、AST、TBIL和ALP)、血清肾功能指标(肌酐、尿素和尿酸)和丙二醛(MDA)含量显著升高。枸杞提取物改善了PHZ注射大鼠血液学指标、促红细胞生成素激素、铁蛋白水平、血清蛋白、肝功能酶、肾功能参数、抗氧化酶活性和MDA含量。综上所述,本研究结果表明,WGO对改善PHZ所致的溶血性贫血和毒性具有治疗和保护作用。
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引用次数: 0
Possible effects of escitalopram on Hao1, Rspo2 and Runx2 gene expression and bone formation in the foetuses of albino rat 艾司西酞普兰对白化大鼠胎儿Hao1、Rspo2、Runx2基因表达及骨形成的可能影响
Pub Date : 1900-01-01 DOI: 10.5455/egysebz.20220214061458
A. Kareim, Walaa M Shaalan, M. Iskandar
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引用次数: 0
Gene expression of the Springtail, Folsomia candida exposed to excess phosphorous in different soils 不同土壤中过量磷对假丝叶虫基因表达的影响
Pub Date : 1900-01-01 DOI: 10.5455/egysebz.20220528075821
M. Ageba, N. Straalen, Basma Assiuty
The gene expression measurements can be used as a biomarker for species functioning under stressed conditions in polluted soil. In this study, the response of genes to excessive phosphorous levels in the soil was assessed. The expression of Folsomia candida’s gene encoding the deduced MTC, Cyp6, and HSP70 was evaluated,using quantitative Rt-PCR. F. candida, was exposed to two different field soils at different distances from two fertilizer factories in Kafr El Zayat and Abou Zaabal in Egypt with different levels of phosphorous concentrations. In addition to exposure to phosphate-amended LUFA soil. Our results indicated that the expression of MTC was significantly decreased after exposure to phosphorus-rich field soils and phosphate-amended LUFA soil, Gene expression of Cyp6 was significantly increased in response to higher phosphorous concentrations in soil. The expression of HSP70 did not change in response to all treatments. Gene expression can be used as an early indicator of excess Phosphorous in soil, which effect on the bioavailability of other important nutrients to improve soil quality assessment.
基因表达测量可作为物种在污染土壤胁迫条件下功能的生物标志物。在本研究中,评估了基因对土壤中磷水平过高的反应。采用定量Rt-PCR技术检测假丝酵母编码MTC、Cyp6和HSP70基因的表达。念珠菌,在埃及Kafr El Zayat和Abou Zaabal两个化肥厂不同距离的两个不同土壤中暴露,磷浓度不同。除了暴露在磷酸盐改良的LUFA土壤中。结果表明,暴露于富磷田间土壤和磷改性LUFA土壤后,MTC基因的表达显著降低,Cyp6基因的表达在高磷土壤中显著升高。HSP70的表达在不同处理下均无明显变化。基因表达可作为土壤磷含量超标的早期指标,影响其他重要养分的生物有效性,提高土壤质量评价。
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引用次数: 0
RNA interference gene silencing of OctßRs genes induced disrupted Drosophila leg muscles architecture RNA干扰基因沉默OctßRs基因诱导果蝇腿部肌肉结构紊乱
Pub Date : 1900-01-01 DOI: 10.5455/EGYSEBZ.20190108102009
B. Afifi, S. Kholy, I. E. Husseiny, A. Seif
Muscular system in most of animals is responsible for their locomotion as well as movement of internal organs under the control of nervous system. Many genes are involved in the process including adrenaline/noradrenaline in vertebrates. Insect muscular system is considered a powerful tool in studying genes involved in this process . Drosophila is an excellent model organism for muscle research due to its well-developed genetics and the availability of techniques to study its muscle structure and physiology. In the present study, reverse-genetic (RNAi) approaches were used to specifically silencing endogenous mRNA of Drosophila OctbetaRs, counterparts of beta adrenergic receptors of vertebrates to define the role of octopamine receptors genes in the maintenance of striated muscle architecture. Results revealed myofibrillar and/or mitochondrial morphologies disruption of leg muscles due to OctsRs gene silencing indicated that those genes are required in adults to maintain normal leg muscle architecture. The present effort indicated that Drosophila is a useful model for studying the abnormalities of skeletal muscles which may leads to specific myopathy found in the human population.
大多数动物的肌肉系统不仅在神经系统的控制下控制内脏器官的运动,而且还负责它们的运动。许多基因参与了这个过程,包括脊椎动物的肾上腺素/去甲肾上腺素。昆虫肌肉系统被认为是研究参与这一过程的基因的有力工具。果蝇是一种很好的肌肉研究模式生物,因为它具有发达的遗传学和可用的技术来研究它的肌肉结构和生理。在本研究中,采用反遗传(RNAi)方法特异性沉默章鱼胺受体的内源性mRNA,以确定章鱼胺受体基因在维持横纹肌结构中的作用。章鱼胺受体是脊椎动物β肾上腺素能受体的对应物。结果显示,OctsRs基因沉默导致腿部肌肉的肌纤维和/或线粒体形态破坏,表明这些基因是维持正常腿部肌肉结构所必需的。目前的努力表明,果蝇是研究骨骼肌异常的一个有用的模型,骨骼肌异常可能导致人类中发现的特定肌病。
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引用次数: 1
Morphological and morphometric insights of two hemiurid trematodes from the greater lizardfish Saurida tumbil (Pisces: Synodontidae) inhabiting the Red Sea 生活在红海的大蜥蜴鱼tumbil(双鱼座:刺尾鱼科)的两种半尾吸虫的形态学和形态计量学研究
Pub Date : 1900-01-01 DOI: 10.5455/egysebz.20191204055415
Rewaida Gaber, Lamia Bakr, Mahy Mallah, S. Maher
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引用次数: 0
Seasonal variations of heavy metals levels in water, sediment and soft parts of edible bivalve Donax trunculus collected from Port Said coast, Egypt 从埃及塞得港海岸采集的可食用双壳类动物多纳克斯(Donax trunculus)的水、沉积物和柔软部位中重金属含量的季节变化
Pub Date : 1900-01-01 DOI: 10.5455/egysebz.20190812013715
F. Heiba, M. Nassef, M. Mona, Esraa El-Rasheedy
The aim of this study was to get quantitative data on the seasonal variations of heavy metals levels (i.e. Fe, Mn, Cu, Zn, Pb and Cd), in different samples (i.e. surface water, sediment and soft parts of the bivalvian Donax trunculus) collected from four consecutive sites over the four seasons; El-Gamil airport, El-Gamil inlet, El-Fardous, and El-Manasra along Port Said Coast, Port Said, Egypt. The relationships between physicochemical parameters (i.e. pH, Temperature, Salinity and D.O) and heavy metal concentrations were assessed, as well. The obtained results showed that the order of mean of the heavy metals concentration in water was appeared as Zn ˃ Fe ˃ Pb ˃ Cd ˃ Cu, while their order in the soft parts of D. trunculus was appeared as Fe˃ Mn ˃Zn˃ Pb ˃Cu˃ Cd. Furthermore, the data rveaed that the mean of heavy metals concentration in sediment samples was appeared as Fe˃ Mn ˃Zn˃ Pb ˃Cu˃ Cd. Additionally, the concentrations of the heavy metals in water samples recorded the lowest value compering to their concentrations in the soft parts of D. trunculus and sediment samples. As a result, Fe recorded its higher value in the soft parts of D. tranculus during spring season which was 854.8, 542.6, 352.7 mg/g dry weight in El-Gamil airport, El-Gamil inlet and El-Fardous sampling sites, respectively. . The obtained data indicated that there was a noticeable relationship between physicochemical parameters (i.e. pH, Temperature, Salinity and D.O) and heavy metal accumulation in surface water, sediment and soft parts of the molluscan D.trunculus Therefore studies on chronic toxic effects of heavy metals in marine organisms, especially edible bivalvia, are needed as a possible public health threat from sea food dietary.
本研究的目的是对连续4个地点采集的不同样品(即双壳甲鱼的地表水、沉积物和软质部分)中Fe、Mn、Cu、Zn、Pb和Cd重金属含量的季节变化进行定量分析;El-Gamil机场,El-Gamil入口,El-Fardous和El-Manasra沿着塞得港海岸,塞得港,埃及。评估了理化参数(pH、温度、盐度和D.O)与重金属浓度之间的关系。结果表明,水中重金属浓度的平均值顺序为Zn - Fe - Pb - Cd - Cu,而龙骨软质部分重金属浓度的平均值顺序为Fe - Mn - Zn - Pb - Cu - Cd。此外,数据还显示,沉积物样品中重金属浓度的平均值顺序为Fe - Mn - Zn - Pb - Cu - Cd。水样中重金属含量最低,低于树干软质部分和沉积物中重金属含量。结果表明,春季时,El-Gamil机场、El-Gamil进水口和El-Fardous取样点的芦花软部铁含量最高,分别为854.8、542.6和352.7 mg/g干重。所获得的数据表明,物理化学参数(即pH值、温度、盐度和D.O)与重金属在地表水、沉积物和软体动物d.t trunculus软部的积累之间存在显著的关系。因此,需要研究重金属对海洋生物,特别是食用双壳类生物的慢性毒性效应,作为海产品饮食中可能存在的公共健康威胁。
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引用次数: 1
Effects of Lactobacillus plantarum on metabolic disorders in streptozotocin-induced diabetic rats 植物乳杆菌对链脲佐菌素诱导的糖尿病大鼠代谢紊乱的影响
Pub Date : 1900-01-01 DOI: 10.5455/egysebz.20211115040111
Wafaa Mohamed, M. Hosny, H. M. A. Shady, A. Askora, E. Hussieny
: Probiotics could have beneficial effects beyond gastrointestinal health, as they were found to improve certain metabolic disorders such as hyperglycaemia and hyperlipidaemia. This study aimed to investigate the effect of probiotic Lactobacillus plantarum on progression of streptozotocin (STZ)-induced type2 diabetes in male rats. The animals were divided into: Normal control (NC); Probiotic control (PC) rats administered Lactobacillus plantarum 10 7 CFU/ml/orally/daily for 28 days; STZ-Diabetic control rats (DC, 50 mg/kg/i.p.); Treated diabetic (TD) rats that received Lactobacillus plantarum . Fasting blood glucose, glycosylated haemoglobin, serum, insulin, blood lipid profile, and cardiac markers (creatine kinase; CK and lactate dehydrogenase; LDH) were recorded. Lactobacillus plantarum administration to diabetic rats prevent body weight decreasing and caused a significant ( P = 0.0001) decrease of serum glucose after the first week by 22.34% from the initial value as compared to diabetic rats. Furthermore, at the end of experiment, we observed a significant ( P < 0.05) decrease in HbA1c (19.1%) and increase ( P = 0.0001) of insulin level in (TD) group by (50.90%) compared to (DC). A significant reduction ( P = 0.0001) in CK (39.32%) and LDH (28.28%), TC (24.27%), TG (44.5%), VLDL-C (44.5%) and LDL-C (36.9%) associated with elevation of HDL-C level (33.52%) was also observed in the (TD) group compared to (DC) group. In conclusion, the probiotic L. plantarum significantly improve the onset of hyperglycaemia, hyperinsulinemia, dyslipidaemia, and may contribute to the improvement of cardiovascular disease risk factors in STZ-induced diabetic rats, indicating a lower risk of diabetes and its complications.
益生菌除了对胃肠道健康有益外,还能改善某些代谢紊乱,如高血糖和高脂血症。本研究旨在探讨益生菌植物乳杆菌对链脲佐菌素(STZ)诱导的雄性大鼠2型糖尿病进展的影响。实验动物分为:正常对照组(NC);益生菌对照(PC)大鼠给予植物乳杆菌10.7 CFU/ml/日口服,连续28天;stz -糖尿病对照大鼠(DC, 50 mg/kg/i.p);用植物乳杆菌治疗糖尿病大鼠。空腹血糖、糖化血红蛋白、血清、胰岛素、血脂和心脏标志物(肌酸激酶;CK和乳酸脱氢酶;记录LDH)。糖尿病大鼠给予植物乳杆菌可防止体重下降,第一周后血糖较糖尿病大鼠降低22.34% (P = 0.0001)。实验结束时,我们观察到(TD)组HbA1c较(DC)组显著(P < 0.05)降低(19.1%),胰岛素水平较(DC)组显著(P = 0.0001)升高(50.90%)。与(DC)组相比,(TD)组CK(39.32%)、LDH(28.28%)、TC(24.27%)、TG(44.5%)、VLDL-C(44.5%)和LDL-C(36.9%)与HDL-C水平升高(33.52%)相关也显著降低(P = 0.0001)。综上所述,植物乳杆菌可显著改善stz诱导的糖尿病大鼠的高血糖、高胰岛素血症、血脂异常的发生,并可能有助于改善心血管疾病危险因素,降低糖尿病及其并发症的发生风险。
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引用次数: 0
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THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Zoology)
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