Effects Of Annona Muricata (Linn) On The Morphology Of Pancreatic Islet Cells Of Experimentally-Induced Diabetic Wistar Rats

The Internet Journal of Alternative Medicine Pub Date : 2007-12-31 DOI:10.5580/229c

D. Adeyemi, O. Komolafe, S. O. Adewole, E. Obuotor, T. Adenowo

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引用次数: 16

Abstract

The study was designed to evaluate the effects of methanolic extract of Annona muricata leaves on the pancreatic islet cells of streptozotocin induceddiabetic rats. Thirty adult Wistar rats were randomly assigned into three groups (A, B and C) of ten rats each. Group A was the control, Group B was untreated diabetic group and group C was A. muricata-treated group. Diabetes mellitus was experimentally induced in groups B and C by a single intra-peritoneal injection of 80mg/kg streptozotocin dissolved in 0.1M citrate buffer. Group A rats were intraperitoneally injected with equivalent volume of citrate buffer. Daily intra peritoneal injection of 100mg/kg A. muricata was administered to group C rats for two weeks. The rats were sacrificed and the pancreas were removed and fixed in Bouins fluid. The tissues were processed for paraffin embedding and sections of 5μm thickness were produced and stained. Histopathological examination of the stained sections showed regeneration β-cells of islets of pancreas of A. muricata-treated rats when compared to untreated diabetic group of rats.

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野牡丹对实验性糖尿病大鼠胰岛细胞形态的影响

本研究旨在探讨番荔枝叶甲醇提取物对链脲佐菌素诱导的糖尿病大鼠胰岛细胞的影响。将30只成年Wistar大鼠随机分为A、B、C三组，每组10只。A组为对照组，B组为糖尿病未治疗组，C组为木田治疗组。B、C组小鼠腹腔单次注射溶于0.1M柠檬酸缓冲液中的链脲佐菌素80mg/kg诱导糖尿病。A组大鼠腹腔注射等体积的柠檬酸缓冲液。C组大鼠每日腹腔注射村田青100mg/kg，连续2周。处死大鼠，切除胰腺，用Bouins液固定。组织进行石蜡包埋处理，制作厚度为5μm的切片并染色。染色切片的组织病理学检查显示，与未治疗的糖尿病组大鼠相比，木参治疗组大鼠胰腺胰岛有再生β细胞。

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The Internet Journal of Alternative Medicine

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