Isolation, Purification and Analysis of Pancreatic Lipase from ‘Gallus gallus domesticus’

B. Sandhya, Nagamani. T.S
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Abstract

This article discusses the isolation of pancreatic lipase enzyme from the pancreas of Gallus gallus domesticus. Whereas lipase catalyses the hydrolysis and the synthesis of esters formed from glycerol and long-chain fatty acids. Lipases occur widely in nature, it involves applications like organic syntheses, hydrolysis of fats, oils, modification of fats, flavor enhancement in food processing, detergent industries, pharmaceutical industries, chemical analyses, and biodiesel production. Pancreatic lipase was purified to the homogeneity by 70% saturated Ammonium sulphate further, it was dialysate using the dialysis membrane and then gel filtration chromatography was carried out by Sephadex G-75 and DEAE cellulose. The molecular weight of purified lipase sample was determined by SDSPAGE, it was found to be 98KDa. The lipase was active in the pH range of 5-10 with an optimum pH of 6.0. The optimum temperature for the hydrolysis of olive oil was 37ºC in the range of 25ºC - 50ºC.
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家鸡胰脂肪酶的分离纯化及分析
本文讨论了从家鸡胰腺中分离胰脂肪酶的方法。而脂肪酶则催化由甘油和长链脂肪酸形成的酯的水解和合成。脂肪酶广泛存在于自然界中,它涉及到有机合成、脂肪水解、油脂改性、食品加工、洗涤剂工业、制药工业、化学分析和生物柴油生产中的风味增强等应用。胰脂肪酶经70%饱和硫酸铵进一步纯化至均匀,透析膜透析后用Sephadex G-75和DEAE纤维素凝胶过滤层析。经SDSPAGE测定所得脂肪酶的分子量为98KDa。脂肪酶在5 ~ 10 pH范围内具有活性,最适pH为6.0。在25 ~ 50℃范围内,橄榄油水解的最佳温度为37℃。
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