{"title":"Ultrasonic characterization of cell culture medium in the temperature range from 25°C to 50°C","authors":"S. A. López-Haro, J. L. Reyes, A. Vera, L. Leija","doi":"10.1109/PAHCE.2012.6233443","DOIUrl":null,"url":null,"abstract":"The aim of this work was the ultrasonic characterization of the cell culture medium used for Madin-Darby canine kidney (MDCK) cells. To design and build a special container for the speed-of-sound (SOS) temperature dependence measurement of the cell culture media was necessary. Pulse-echo technique and a step reflector were used to obtain the temperature dependence of SOS results. Water and cell culture media SOS are compared; both liquids behavior show an upward tendency in the temperature range studied. Culture media SOS results were fitted by using both a first and a second order curve; second order curve had better correlation coefficient than the other one. Speed-of-sound varies from 1510 m/s at 25°C to 1540 m/s at 50°C.","PeriodicalId":255935,"journal":{"name":"2012 Pan American Health Care Exchanges","volume":"43 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2012-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"2012 Pan American Health Care Exchanges","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/PAHCE.2012.6233443","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 2
Abstract
The aim of this work was the ultrasonic characterization of the cell culture medium used for Madin-Darby canine kidney (MDCK) cells. To design and build a special container for the speed-of-sound (SOS) temperature dependence measurement of the cell culture media was necessary. Pulse-echo technique and a step reflector were used to obtain the temperature dependence of SOS results. Water and cell culture media SOS are compared; both liquids behavior show an upward tendency in the temperature range studied. Culture media SOS results were fitted by using both a first and a second order curve; second order curve had better correlation coefficient than the other one. Speed-of-sound varies from 1510 m/s at 25°C to 1540 m/s at 50°C.
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