The Degree of Hemagglutination on a Plastic Microfluidic Chip Using Impedimetric Detection

Abstract

Hemagglutination is a specific form of agglutination that involves red blood cells (RBCs) and antibody used for blood typing testing. Some conventional testing of blood typing has a restriction in manual observation. In this study, a simple prototype of a disposable plastic microfluidic chip coupled with a pair of coplanar electrodes made of titanium resistant wires was fabricated to investigate the degree of agglutination based on electrical impedimetric measurement. The charge transfer resistance in the impedimetric detection was used for the analysis of agglutination level. This proposed approach provided quantitative classification of agglutination level from non-agglutination (level 0) to strong agglutination (level 4+) with small amount of whole blood sample (43% Hematocrit).