Single Cell Position Determination and Transformation from Static High-resolution Digital Image to Laser-microdissector Coordinate System Using Image Processing Techniques

Abstract

Such laser microdissection design is discussed that requires no human interaction and yet capable of μm accuracy. The goal is to boost speed, accuracy, and smooth workflow between slide scanning and laser cutting of suspicious areas from tissue sample based on software evaluation of the digital slides. Fully automated, precise, robust solution has been developed using hybrid method that includes static matrix transformation for preliminary position calculation, computer vision, and image processing technique for real-time object detection and position adjustment. Software implementation also tested on microdissector equipment. Applying this methodology in clinical pathology could reduce the waiting lists, the overload of clinics, laboratory technicians.