Thiamine, thiamine phosphates and thiamine metabolizing enzymes in synaptosomes of rat brain.

Basic and applied histochemistry Pub Date : 1990-01-01
U Laforenza, C Patrini, P Mazzarello, M Poloni, G Rindi
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Abstract

Thiamine and thiamine mono-, pyro- and triphosphate were found at detectable levels in synaptosomes isolated from whole rat brain. Synaptosomes prepared from whole brain, cerebellum and medulla were also found to contain uridine and inosine mono- and diphosphatases as well as the thiamine pyrophosphate synthetizing and hydrolyzing enzymes, but no thiamine monophosphatase. By isoelectric focusing on thin layer polyacrylamide gel of Triton X-100 homogenates of synaptosomes, thiamine pyrophosphatase activity could be separated into 10 bands with different isoelectric points. The contents of thiamine compounds and enzymes in synaptosomes were generally lower than those found in neuronal cell bodies.

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大鼠脑突触体中硫胺素、硫胺素磷酸和硫胺素代谢酶的研究。
从大鼠全脑分离的突触体中发现了可检测的硫胺素、单磷酸硫胺素、热磷酸硫胺素和三磷酸硫胺素。从全脑、小脑和髓质制备的突触体中也发现含有尿苷和肌苷单磷酸酶和二磷酸酶,以及硫胺素焦磷酸合成和水解酶,但不含硫胺素单磷酸酶。通过等电聚焦Triton X-100突触体匀浆的薄层聚丙烯酰胺凝胶,可以将硫胺素焦磷酸酶活性分为10个不同等电点的条带。突触体中硫胺素化合物和酶的含量普遍低于神经元细胞体。
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Histochemical studies on irradiated and obstructive human submandibular glands. Protein distribution and lectin-binding in the degenerative glands. Cytochemical evaluation of C-heterochromatic-DNA in metaphase chromosomes. Integrated dystrophin analysis using immunocytochemical, biochemical and genetic techniques. Substance P- and calcitonin gene-related peptide-like immunoreactive pericellular baskets in human trigeminal ganglion. Phenylfluorone, a new staining agent with higher selectivity for tin.
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