Applicability of ion mobility spectrometry for detection of quarantine pests in wood

K. Ewing, J. Sanghera, S. Myers, A. Ervin, C. Carey, G. Gleason, L. Mosser, L. Levy, M. Hennessey, R. Bulluck
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Abstract

Visual inspection is the most commonly used method for detecting quarantine pests in agricultural cargo items at ports. For example, solid wood packing material (SWPM) at ports may be a pathway for wood pests and is a frequent item of inspection at ports. The inspection process includes examination of the external surface of the item and often destructive sampling to detect internal pest targets. There are few tools available to inspectors to increase the efficiency of inspection and reduce the labor involved. Ion mobility spectrometry (IMS) has promise as an aid for inspection. Because pests emit volatile organic compounds (VOCs) such as hormone like substances, Ion Mobility Spectrometry (IMS) was investigated for possible utility for detecting pests during inspection. SWPM is a major pest pathway in trade, and fumigation of many kinds of wood, including SWPM, with methyl bromide (MeBr) following a published schedule1 is regularly conducted for phytosanitary reasons prior to shipment to the United States. However, the question remains as to how long the methyl bromide remains in the wood samples after fumigation such that it could act as an interferent to the detection of pest related VOC emissions. This work investigates the capability of ion mobility spectrometry to detect the presence of residual methyl bromide in fumigated maple and poplar wood samples at different times post fumigation up to 118 days after fumigation. Data show that MeBr can be detected in the less dense poplar wood up to 118 days after fumigation while MeBr can be detected in the denser maple wood 55 days after fumigation.
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离子迁移谱法检测木材中检疫性害虫的适用性
目视检验是口岸农业货物检疫性有害生物检测最常用的方法。例如,港口的实木包装材料(SWPM)可能是木材害虫的途径,也是港口经常检查的项目。检查过程包括检查物品的外表面,经常进行破坏性取样以检测内部害虫目标。检查员很少有工具可以提高检查效率并减少所涉及的劳动力。离子迁移率谱法(IMS)是一种很有前途的检测手段。由于害虫会释放出挥发性有机化合物(VOCs),如激素类物质,因此研究了离子迁移谱法(IMS)在检测害虫过程中的可能应用。SWPM是贸易中主要的有害生物途径,出于植物检疫原因,在运往美国之前,按照公布的附表1定期对包括SWPM在内的多种木材进行甲基溴熏蒸。然而,问题仍然是,熏蒸后甲基溴在木材样品中存留多久,从而可以干扰检测与害虫有关的挥发性有机化合物排放。本研究研究了离子迁移谱法在熏蒸后不同时间(熏蒸后118天)检测枫木和杨木样品中残留甲基溴的能力。数据显示,在熏蒸后118天,密度较低的杨木中可以检测到MeBr,而在熏蒸后55天,密度较高的枫木中可以检测到MeBr。
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