Apoptosis in the liver of db/db Mice Female in Postnatal Ontogenesis

Abstract

Immunohistochemical analysis and morphometric evaluation of the expression of antiapoptotic Bcl-2 protein and proapoptotic protein Bad in the liver cells of homozygous mice BKS.Cg-Dock7m+/+Leprdb/J (db/db mice) females at different periods of postnatal ontogeny (at the age of 8 and 16 weeks) in the development of obesity and diabetes mellitus type 2 (DM2) were done. In the liver of db/db mice at the age of 8 weeks weak Bcl-2 and Bаd-positive signals were detected in the heterogeneous population of sinusoidal cells of the of liver blood capillaries and in single hepatocytes. It was found that immunohistochemical staining areas of both proteins were increased in mice aged 16 weeks with DM2. However in adult animals, Bcl-2 expression area was almost 2,5 times higher than this parameter value for Bad. This fact indicates the antiapoptotic protection of liver cells and the creation of conditions for blocking the development of apoptosis mitochondrial "branch" at non-alcoholic fatty liver disease (NAFLD) in db/db mice postnatal ontogenesis.