Optimization of the Factors that Enhance the Isolation Efficiency of Collagen from Pangasius Fish Skin

Abstract

This research aimed to determine the factors that enhance the extraction efficiency of collagen from pangasius fish skin. It conducted the fish skin purification method by LasNa 0.5% in NaOH 1% during 8 hours, and then the collagen was isolated from purified fish skin by acetic acid. At the same time, the optimization method was used to describe the extraction process of collagen with the objective function (y) and three factors which affected the objective function were the ratio of acetic acid to fish skin (x1), concentration of acetic acid (x2), and extraction time of collagen (x3). The quadratic polynomial optimization model y has been used to create response surface for extraction efficiency of collagen. Under these conditions: the ratio of acetic acid to fish skin was 13/1, the concentration of acetic acid was 0.58M and the extraction time was 26 hours, the collagen extraction efficiency achieved maximum value of 72.9%. This result was the scientific basis to choose the extraction conditions in technology producing collagen from fish skin in order to get the high quality product. The final product had white colour, soft fibre structure to meet the collagen quality in food.